Abstract
This protocol serves to determine the integrity of the barrier function of (murine) epidermis. Defects in the barrier function lead to dehydration and infection in neonatal mice/humans. One possible way to assess epidermal barrier integrity is by a dye penetration assay as described hereunder. This assay should be done on unfixed, untreated tissues (e.g. formaldehyde- or glutaraldehyde-fixed) or on whole mouse embryos (E18.5). This protocol was adapted from Hardman (1998).
Keywords: Skin integrity, Permiability, Dye penetration assay
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This protocol was adapted from Hardman et al. (1998). Our work is supported by the Dutch Cancer Society (RUG 2012-5549), Stichting Kinder Oncologie Groningen (SKOG) and EMBO grants to FF.
References
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