Abstract
Pollen tube is regarded as an excellent single-cell model system in plant cell studies. This protocol describes the use of a rapid and reliable immunofluorescence labeling method for studying in situ localization of proteins in pollen tubes. The whole experiment contains two major steps: pollen tube in vitro germination, and pollen tube fixation and immunolabeling. It takes about 2 days from pollen tube germination to immunofluorescence detection.
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
The original version of this protocol was described in Wang et al. (2010). This work was supported by grants from the Research Grants Council of Hong Kong (CUHK 466011, 465112, 466613 and CUHK2/CRF/11G) to L.J.
References
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