Abstract
Anti-virulence agents against MRSA inhibit the production of disease-causing virulence factors, such as alpha-hemolysin, but are neither bacteriostatic nor bactericidal. Here we discuss a rapid method to screen for MRSA anti-virulence agents by measuring alpha-hemolysin production through ELISA. This protocol can be used with other alpha-hemolysin producing bacteria or for other excreted toxins to which antibodies exist.
Materials and Reagents
Equipment
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Procedure
Recipes
Acknowledgments
We thank Barbara Truitt and Dr. Michelle Pesho for their contributions to the development of this protocol. This protocol has been adapted from John R Crowther, (2000). Funding for this work was provided by a Grant-in-Aid from the American Heart Association, Great Rivers Affiliate, and by a grant from the Steris Foundation.
References
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