Abstract
Two critical steps when investigating an animal model of a virus infection are consistently successfully infecting animals and accurately determining viral titers in tissue throughout the course of infection. Here we discuss in detail how to infect mice with SARS-CoV and then quantify the titer of virus in the lung.
Keywords: SARS-CoV, Virus titration, Virus infection, Lung preparation
Materials and Reagents
Equipment
Procedure
Recipes
Acknowledgments
This work was supported by grants from the National Institutes of Health (PO1AI060699, RO1AI091322). The protocol described herein was based on the following manuscript: Zhao et al. (2010).
References
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