Abstract
Astrovirus are small, nonenveloped, single-stranded RNA viruses that cause diarrhea in a wide variety of mammals and birds. Here, we describe astrovirus propagation, purification and titration. The Caco-2 human intestinal adenocarcinoma cell line is most widely used for studying astrovirus, although other cell lines, such as 293, T84 and LLC-MK2 can be used for propagation. However, Caco2 cells are desirable for their ability to form a differentiated intestinal epithelium, mimicking the human intestine and providing a realistic model for astrovirus growth and propagation.
Keywords: Astrovirus, Propagation, Purification, Titration, Intestine
Materials and Reagents
Equipment
Procedure
Notes
Recipes
Acknowledgments
This protocol was adapted from the previous publications DuBois et al. (2013); Moser and Schultz-Cherry (2008); and Willcocks et al. (1990). Funding for this research was provided by the Children’s Infection Defense Center, the Hartwell Foundation, and the American Lebanese Syrian Associated Charities and St Jude Children’s Research Hospital.
References
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