Abstract
The method described here allows measuring the effect of exogenously introduced modifications to in vitro-transcribed mRNA on the translation in cells. Using cells derived from knockout mice and control littermates, this method enables to compare the results in the presence or absence of specific gene products. In our lab, we used this protocol to check whether the exogenous addition of 5’ capping and 2’-O methylation to in vitro-mRNA affects the translational efficiency. Here we describe the details of our experiments.
Materials and Reagents
Equipment
Procedure
Acknowledgments
This work was adapted from the following paper: Kimura et al. (2013). This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology, the Japan Science and Technology Agency, and by the Ministry of Health, Labour and Welfare.
References
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