Abstract
The Stat (Signal Transducer and Activator of Transcription) family of proteins are critical signal transducers involved in fundamental cellular processes, including cell growth and differentiation, development, apoptosis, immune responses and inflammation. In here, we describe a simple and reproducible flow cytometry protocol to measure Stat protein phosphorylation in splenocyte preparations from malaria infected mice.
Materials and Reagents
Equipment
Procedure
Acknowledgments
This protocol is adapted from Villegas-Mendez et al. (2013).
References
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