Abstract
Water use efficiency (WUE) is a quantitative measurement of how much biomass or yield is produced per amount of water used. It is an important physiological factor for agriculture, especially in areas with a limited accessibility of water. It is also crucial in a better understanding of drought tolerance and drought resistance. The most common method to measure the WUE of individual plants is to weigh each pot or container to monitor the water loss and to harvest and measure biomass or yield at the end of the experiment. Since water can be lost from the soil surface through evaporation, there is a need to perform WUE measurements in a closed system. Here, we describe a simple method for WUE determination for Arabidopsis thaliana.
Keywords: Water use efficiency (WUE), Arabidopsis thaliana, Biomass, Plants, Falcon tubes
Materials and Reagents
Equipment
Procedure
Acknowledgments
Please also cite the following publication when citing this protocol: Wituszyńska et al. (2013). This work was supported by the Welcome/2008/1 Program operated within the framework of the Foundation for Polish Science and co-financed by the European Regional Development Fund.
References
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