Abstract
This method is used for making high titer human immunodeficiency virus type-1 (HIV-1) virus stock for subsequent infection assays. The amplification of T-tropic HIV-1 virus (IIIB strain) uses the CD4+ T cell line H9.
Materials and Reagents
Equipment
Procedure
Day 1
Notes
The experiment using HIV-1 needs to be done in a BSL-3 lab and the personnel require special training for working with HIV-1 virus.
Recipes
Acknowledgments
The author thanks Dutschman, GE and Dr. Yung-Chi Cheng at the Department of Pharmacology, Yale School of Medicine for the adaptation of this method and their support on the research of anti-HIV-1 drug development.
References
If you have any questions/comments about this protocol, you are highly recommended to post here. We will invite the authors of this protocol as well as some of its users to address your questions/comments. To make it easier for them to help you, you are encouraged to post your data including images for the troubleshooting.