Abstract
DNA methylation is the most studied epigenetic modification, which involves the addition of a methyl group to the carbon-5 position of cytosine residues in DNA. DNA methylation is important for the regulation of gene expression. Bisulfite sequencing is the gold standard technique for determining genome-wide DNA methylation profiles in eukaryotes. This protocol describes how to prepare libraries of genomic DNA for whole-genome bisulfite sequencing in Arabidopsis, which could be adapted for use in other plant species.
Materials and Reagents
Equipment
Procedure
The overall workflow of the procedure is illustrated in Figure 1. Figure 1. Workflow of library construction for genome-wide bisulfite sequencing
Recipes
Acknowledgments
The protocol was adapted from Wang et al. (2013). This work was supported by a grant from the National Science Foundation (IOS-0842716) awarded to Z.M.
References
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