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Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA

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Bio-protocol publishes high quality, step-by-step protocols across the life sciences.

Biochemistry Biophysics Cancer Biology Cell Biology Developmental Biology Immunology Microbiology Molecular Biology Neuroscience Plant Science Stem Cell Systems Biology

Recognizing the need for greater reproducibility in scientific research, our collaborating journals encourage their
authors to publish step-by-step protocols in Bio-protocol.

Gregory Finnigan

Professor at Kansas State University

"The reviews we received from Bio-protocol were very insightful and greatly improved the clarity of our protocols."

Murugappan Sathappa

Research scientist at the Broad Institute of MIT and Harvard

"I truly appreciate the mission of Bio-protocol to publish protocols that can be reproduced by others."

Meenal Sinha

Associate specialist at UCSF

"Reviewing for Bio-Protocol has been very rewarding on multiple levels. The Bio-Protocol team is very professional and efficient."

Archive: Triple Fluorescence Anisotropy Reporter Imaging in Living Cells http://bio-protocol.org/e3226 #cellimaging #protocol @eLife

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Archive: Inducing Alcohol Dependence in Rats Using Chronic Intermittent Exposure to Alcohol Vapor http://bio-protocol.org/e3222 #neuroscience #protocol #JNeurosci

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Archive: Determination of Root Exudate Concentration in the Rhizosphere Using 13C Labeling http://bio-protocol.org/e3228 #plantsci #protocol

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