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Measurement of Oxygen Consumption Rate (OCR) and Extracellular Acidification Rate (ECAR) in Culture Cells for Assessment of the Energy Metabolism
测定培养细胞中的消氧率(OCR)和细胞外酸化率(ECAR)以评估能量代谢
作者:Birte Plitzko and Sandra Loesgen日期:05/20/2018,浏览量:6905,Q&A: 0
Mammalian cells generate ATP by mitochondrial (oxidative phosphorylation) and non-mitochondrial (glycolysis) metabolism. Cancer cells are known to reprogram their metabolism using different ...
Sulforhodamine B (SRB) Assay in Cell Culture to Investigate Cell Proliferation
利用磺酰罗丹明B(SRB)法检测细胞的增殖
作者:Esteban A. Orellana and Andrea L. Kasinski日期:11/05/2016,浏览量:20823,Q&A: 0
The SRB assay has been used since its development in 1990 (Skehan et al., 1990) to inexpensively conduct various screening assays to investigate cytotoxicity in cell based studies (Vichai ...
BODIPY 493/503 Staining of Neutral Lipid Droplets for Microscopy and Quantification by Flow Cytometry
显微镜观察BODIPY 493/503 染色中性脂滴以及用流式细胞仪量化
作者:Bo Qiu and M. Celeste Simon日期:09/05/2016,浏览量:19186,Q&A: 0
Lipid droplets (LDs) are ubiquitous, dynamic organelles and function as a storage depot for neutral lipids, including triglycerides and cholesterol esters (Walther and Farese, 2012). The movement of ...
Superoxide Dismutase (SOD) and Catalase (CAT) Activity Assay Protocols for Caenorhabditis elegans
秀丽隐杆线虫中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性测定实验方案
作者:Jing Zhang, Rui Chen, Zhenyang Yu and Lili Xue日期:08/20/2017,浏览量:8501,Q&A: 0
Assays for superoxide dismutase (SOD) and catalase (CAT) activities are widely employed to indicate antioxidant responses underlying the toxic effects of test chemicals. Yet, earlier studies mainly ...
Analysis of Protein Stability by the Cycloheximide Chase Assay
放线菌酮追踪检测分析蛋白质的稳定性
Comparison of protein stability in eukaryotic cells has been achieved by cycloheximide, which is an inhibitor of protein biosynthesis due to its prevention in translational elongation. It is broadly ...
Microbial Mutagenicity Assay: Ames Test
微生物诱变分析:Ames试验
作者:Urvashi Vijay, Sonal Gupta, Priyanka Mathur, Prashanth Suravajhala and Pradeep Bhatnagar日期:03/20/2018,浏览量:5763,Q&A: 2
The Microbial mutagenicity Ames test is a bacterial bioassay accomplished in vitro to evaluate the mutagenicity of various environmental carcinogens and toxins. While Ames test is used to ...
Measurement of the Intracellular Calcium Concentration with Fura-2 AM Using a Fluorescence Plate Reader
使用荧光读板仪通过钙指示剂Fura-2 AM 测定细胞内钙浓度
作者:Magdiel Martínez, Namyr A. Martínez and Walter I. Silva日期:07/20/2017,浏览量:8257,Q&A: 0
Intracellular calcium elevation triggers a wide range of cellular responses. Calcium responses can be affected or modulated by membrane receptors mutations, localization, exposure to ...
Sucrose Preference Test to Measure Stress-induced Anhedonia
蔗糖偏好实验测量小鼠的应激诱导快感缺乏
作者:Andrew L. Eagle, Michelle Mazei-Robison and Alfred J. Robison日期:06/05/2016,浏览量:14995,Q&A: 2
The 2-bottle choice procedure for assessing sucrose preference is a useful test to investigate anhedonia (i.e., inability to feel pleasure) in laboratory rodents, particularly in ...
Proximity Ligation Assay (PLA) to Detect Protein-protein Interactions in Breast Cancer Cells
邻位连接技术(PLA)检测乳腺癌细胞中蛋白质的相互作用
作者:Mike Z Lin, Janet L Martin and Robert C Baxter日期:05/20/2015,浏览量:14583,Q&A: 0
Protein-protein interaction networks provide a global picture of cellular function and biological processes, and the dysfunction of some interactions causes many diseases, including cancer. The in ...
Hemagglutination Inhibition (HI) Assay of Influenza Viruses with Monoclonal Antibodies
单克隆抗体介导的流感病毒血细胞凝集抑制(HI)试验
Heamagglutination is inhibited when antibodies are present because antibodies to the influenza virus will prevent attachment of the virus to red blood cells. The highest dilution of antibody that ...