Materials and Reagents

Note: Catalog numbers for specific suppliers that we use are given for many of the reagents listed below; an equivalent quality reagent from an alternative supplier can be substituted, with comparable results. Standard materials and reagents for cell culture are required and are not explicitly listed below. Reagents are listed in the approximate order of use in the protocol.

Culture tubes (VWR, catalog number: 60818-667)
50 ml centrifuge tubes (VWR, catalog number: 89039-656)
1.5 ml centrifuge tubes (VWR, catalog number: 20170-038)
Lysing Matrix B, 2 ml tubes (MP Biomedical, catalog number: 116911050)
2 ml centrifuge tubes (VWR, catalog number: 20170-170)
C18 Sep-Pak (Waters, catalog number: WAT051910)
Omix C18 tips (Agilent, catalog number: A57003100)
pH indicator paper
Acinetobacter baumannii (A. baumannii) ATCC 17978 strain
Luria-Bertani (LB) broth (Fisher, catalog number: BP1426)
Na₂S (Sigma, catalog number: 407410)
Na₂HPO₄(Fisher, catalog number: S374-500)
KH₂PO₄ (Fisher, catalog number: P285-500)
KCl (Sigma, catalog number: 7300-500GM)
NaCl (Macron, catalog number: 7581-06)
2-Morpholinoethanesulfonic acid, free acid (MES) (GoldBio, catalog number: M-095-1)
Liquid nitrogen
Bradford reagent (Thermo, catalog number: 23238)
Bovine serum albumin (BSA) (Sigma, catalog number: A4503)
3 kDa cut-off concentrators (Millipore Sigma, catalog number: UFC500396)
Urea (Fisher, catalog number: U15)
Ammonium bicarbonate (ABC) (J.T. Baker, catalog number: 3003-01)
EZ-Link^TM Iodoacetyl-PEG2-Biotin (Biotinylated iodoacetamide, BIAM) (Thermo, catalog number: 21334)
Sequencing grade trypsin (Promega, catalog number: V5117)
Avidin acrylamide resin (Thermo, catalog number: 53151)
Tris(2-carboxyethyl)phosphine (TCEP) (ChemImpex, catalog number: 23004)
Iodoacetamide (IAM) (Sigma, catalog number: I1149)
Trichloroacetic acid (TCA) (Sigma, catalog number: 91228)
Diluted solutions of organic acid (0.1-10%) commonly used in mass spectrometry, e.g., trifluoroacetic acid or formic acid
LC-MS grade acetonitrile (OmniSolv, catalog number: AX0156)
LC-MS grade water (OmniSolv, catalog number: WX0001-1)
Na₂S stock (see Recipes)
Phosphate-buffered saline (PBS) (see Recipes)
Lysis buffer (see Recipes)
Sep-Pak Aqueous Buffer (see Recipes)
Sep-Pak Organic buffer (see Recipes)

Equipment

Note: Typical biochemistry lab equipment is required; therefore, it is not explicitly listed below. Catalog numbers are given for most of the equipment as examples; instruments from alternative manufacturers may be substituted, provided equivalent functionality. Equipment is listed in the approximate order of use in the protocol.

Shaker
Spectrophotometer and cuvettes
Various centrifuges with the appropriate rotors capable of centrifuging a variety of volumes ranging from 1.5 ml to 50 ml or greater (depending on the cell culture volume)
Water bath
Bead Ruptor 12 Homogenizer (Omni, model: 19-050A)
Anaerobic chamber (Vacuum Atmospheres Company, model: HE-453-2-245)
Rotor-Barnstead Thermolyne Labquake Shaker (Barnstead International, model: 400110)
Vacuum manifold (Thermo Fisher Scientific, model: 60104-232)
ISS 110 SpeedVac System (Thermo Fisher Scientific, model: ISS 110)
Trapping column: Symmetry C18 trap column (180 μm × 20 mm, 5 μm)
Analytical column: nanoACQUITY HPLC HHS T3 analytical column (75 μm × 150 mm, 1.8 μm)
Orbitrap Fusion^TM Lumos^TM Tribrid^TM Mass Spectrometer (Thermo Fisher Scientific) interfaced with a nano-flow liquid chromatography (LC) system (e.g., Thermo Scientific EASY-nLC 1200)

Software

UniProt
Proteome Discoverer 2.1 (Thermo Scientific)

Procedure

Cell culture
1. Streak A. baumannii ATCC 17978 onto LB agar plates from frozen glycerol stocks and incubate at 37°C overnight.
2. Use single colonies to inoculate 3 ml LB broth and shake at 37°C overnight.
  Note: The experiment should be performed in biological triplicate and A. baumannii is grown at a liquid-to-air ratio of 1:5, e.g., 10 ml LB in a 50-ml centrifuge tube.
3. Dilute the overnight cultures 1:50 in 10 ml LB and grow for approximately 1 h until OD₆₀₀ = 0.1, at which time the culture should be diluted 1:100 into sterilized flasks containing 250 ml LB and shaken at 37°C until OD₆₀₀ = 0.2.
  Note: Six samples will be collected in total; a biological triplicate of wild-type (WT) untreated and a biological triplicate of WT treated with Na₂S. The volume of culture required depends on the organism of choice and how much protein is needed for the workflow. Typically, 2 mg total protein is required for each replicate but more may be necessary depending on the sensitivity of the MS instrument used.
4. Add Na₂S to three of the six cultures at a final concentration of 0.2 mM and incubate for an additional 30 min at 37°C. Then proceed with the following steps.
5. Centrifuge all cultures at 4,226 × g for 15 min at 4°C, discard the supernatant, and place the pellets on ice.
  Note: Untreated cultures should be centrifuged when OD₆₀₀ = 0.2 and Na₂S-treated cultures should be centrifuged 30 min post-addition of Na₂S.
6. Wash cell pellets with 5 ml cold PBS, centrifuge at 4,226 × g for 15 min at 4°C, and discard the supernatant.
7. Wash the cell pellets with 1 ml cold PBS, transfer to 1.5-ml Eppendorf tubes, centrifuge at 19,090 × g for 3 min, and discard the supernatant.
  Note: Cell pellets can be stored at -80°C until ready to proceed to lysis.

Cell Lysis
Note: Step B1 should utilize buffer that has been degassed, purged with argon, and stored in an anaerobic chamber. If an anaerobic chamber is not available, prepare fresh buffer each time and degas the buffer thoroughly and purge with argon. Steps B3 and B5 are performed in an anaerobic chamber. The aliquot used in Step B4 can be removed from the chamber to complete the assay.
1. Resuspend the pellets in 1 ml Lysis Buffer (see Recipes) and freeze/thaw 5 times in liquid nitrogen and a 37°C water bath.
  Note: Lysis buffer can be modified, e.g., detergent, salt, pH, etc. It is important to make sure that the buffer is degassed and purged with argon (preferably stored in an anaerobic chamber) to minimize exposure to oxygen during Steps B1-B2, which take approximately 1 h.
2. Transfer the lysate to Matrix B lysing tubes and shake on a homogenizer for 45 s (5 s on, 90 s off intervals) at 6.8 m/s and 4°C.
3. Transfer the samples to an anaerobic chamber (<1 ppm O₂) and centrifuge using a standard microcentrifuge for 15 min at 4°C. Transfer the supernatant to a clean 1.5-ml Eppendorf tube, centrifuge again, and transfer to a clean tube to ensure that no cellular debris is present.
4. Remove 2-10 μl supernatant and perform a standard Bradford assay to determine the protein concentration using bovine serum albumin as a protein standard.
5. Based on the Bradford assay, aliquot a volume of lysate that corresponds to 6 mg protein for each replicate. The remaining lysate can be flash frozen and stored at -80°C until whole proteome analysis (Procedure E).

Alkylation and tryptic digestion
Note: Steps C1-C3 should be performed in an anaerobic chamber. If one is not available, use buffer prepared according to the note in Procedure B and minimize sample exposure to oxygen. Alternative buffer exchange protocols may be substituted to reduce time.
1. Buffer exchange the samples from Step B5 in the anaerobic chamber using a 3 kDa cut-off concentrator by repeated cycles of the addition of 8 M urea in 100 mM ABC followed by centrifugation at 4°C for 10 min. Repeat this process 7-8 times. When complete, transfer the sample to a clean 1.5-ml tube, by inverting the concentrator, and centrifuge for 30 s.
  Optional: 8 M urea in 100 mM ABC can be added to increase the sample volume (no more than 500 µl total is recommended).
  Note: Other methods of buffer exchange may be used.
2. Add 2 mg BIAM at room temperature protected from light and incubate for 90 min.
3. Buffer exchange the samples as described previously (Step C1) using 100 mM ABC, until the concentration of urea is approximately 1 M (or less), and transfer to a clean tube.
  Optional: Additional buffer may be added to increase the sample volume (no more than 500 µl is recommended).
4. Remove the samples from the anaerobic chamber, add 60 µg trypsin, and check that the pH of the samples is basic by adding 1 μl sample to pH indicator paper; add an additional 100 mM ABC buffer to adjust the pH if needed. Incubate the samples in a 37°C water bath for 18 h.
5. Desalt the peptides and remove undigested proteins by running the C18 Sep-Pak protocol.
  1. Sample Preparation
    1. Acidify the samples using 10% solutions of trifluoroacetic acid or formic acid to a final concentration of 0.2% and check that the pH is acidic using pH indicator paper; if not acidic, add more acid.
    2. Pellet the insoluble material using a microcentrifuge operating at 16,363 × g for 15 min at 4°C and transfer the supernatant to a clean 1.5-ml tube.
  2. Sep-Pak Preparation
    1. Using a clean razor blade, trim the long end of the Sep-Pak down to the cartridge.
    2. Trim the short side half-way down; this short side is where the syringe will be attached.
  3. Condition the Sep-Pak by passing 20 ml organic buffer (see Recipes) followed by 20 ml aqueous buffer (see Recipes) using a 20-ml syringe.
    Note: Ensure that air is not pulled or pushed through the cartridge here or at any subsequent step.
  4. Apply the sample using a 3-ml syringe, rinse the sample tube with 1 ml aqueous buffer, and apply to cartridge.
  5. Wash the cartridge with 30 ml aqueous buffer.
  6. Elute the peptides from the cartridge using 1.6 ml organic buffer.
  7. Dry the samples in a SpeedVac concentrator and resuspend in 500 μl 100 mM ABC.

Streptavidin enrichment and elution of persulfide-containing peptides
1. Centrifuge 1 ml avidin acrylamide resin at 845 × g for 1 min. Discard the supernatant.
  Note: Resin is in 50% buffer, so 2 ml is required to achieve 1 ml total resin. Determine the volume of resin required for the amount (in mg) of protein used for each sample, according to the manufacturer’s specifications.
2. Equilibrate the resin three times by resuspending in 1 ml 100 mM ABC, centrifuging at 845 × g for 1 min, and removing the supernatant.
3. Add the resuspended peptides (Step C5 above) to the resin and incubate at room temperature for 1 h on a rotor.
4. Wash the resin with 75 ml 2 M NaCl in 100 mM ABC, warmed in a 37°C water bath, using a vacuum manifold.
5. Wash the resin with 15 ml 100 mM ABC warmed in a 37°C water bath.
6. Resuspend the resin in 1 ml 100 mM ABC, transfer to a clean 1.5-ml tube, and add TCEP to a final concentration of 20 mM. Incubate at 37°C on a rotor for 1 h. Centrifuge at 845 × g for 1 min and transfer the supernatant to a clean tube.
7. Repeat Step D6 and combine the supernatants as the eluent.
8. Add 37 mg IAM to the 2 ml combined eluent from Steps D6-D7 above and incubate at room temperature protected from light for 45 min.
9. Use a C18 Omix tip to concentrate and desalt the peptides using a standard protocol and analyze by LC-MS/MS (Procedure F).

Whole proteome analysis for data normalization
1. Dry 25-μg portions of protein from lysates (Procedure B) in a SpeedVac concentrator.
2. Resuspend proteins in 100 μl 8 M urea in 100 mM ABC containing 50 mM IAM and 5 mM TCEP and incubate at room temperature in the dark for 45 min.
3. Precipitate proteins by adding 25 μl 100% TCA (20% (v:v)) and place the samples at -20°C for 1 h. Subsequently, centrifuge the samples at 19,090 × g for 20 min at 4°C and discard the supernatant.
4. Wash the pellets with 500 μl cold acetone and centrifuge again at 19,090 × g for 20 min at 4°C.
5. Dry the samples in a SpeedVac concentrator, resuspend in 10 μl 8 M urea in 100 mM ABC, dilute to 1 M urea with 100 mM ABC, and digest overnight at 37°C by adding a 1:100 (w/w) ratio of trypsin.
6. Desalt the peptides with a C18 Omix Tip using a standard protocol and analyze by LC-MS/MS (Procedure F).

Mass spectrometry
1. Inject 1 μg peptides in 0.1% formic acid into the HPLC system and load onto a Symmetry C18 trapping column (180 μm × 20 mm, 5 μm).
2. Elute the peptides using a nanoACQUITY HPLC HHS T3 analytical column (75 μm × 150 mm, 1.8 μm) with an acetonitrile-based gradient (Solvent A: 0% acetonitrile, 0.1% formic acid; Solvent B: 80% acetonitrile, 0.1% formic acid) at a flow rate of 400 nl/min. Use a 90-min gradient as follows: 0-0.5 min, 0-14% B; 0.5-72 min, 14-50% B; 72-74 min, 50-100% B; 74-77 min, 100% B; 77-78 min, 100-0% B; 78-90 min, isocratic flow at 0% B.
3. Carry out the electrospray ionization with a nanoESI source at a capillary temperature of 260°C and a spray voltage of 1.8 kV.
4. Operate the Orbitrap Fusion instrument in data-dependent acquisition mode with a mass range of 350-2,000 m/z. Select the precursor ions for tandem mass (MS/MS) analysis in Orbitrap, with a 3-s cycle time using HCD at 35% collision energy. Set the intensity threshold at 5e3 and the dynamic exclusion with a repeat count of 1 and an exclusion duration of 30 s.
5. Search the resulting data against the A. baumannii ATCC 17978 database (UniProt UP000094982, 3,780 entries) in Proteome Discoverer 2.1. Allow trypsin digestion specificity with two missed cleavages. Set the mass tolerance for precursor and fragment ions to 10 ppm and 0.6 Da, respectively. Set the carbamidomethylation (CAM) of cysteine residues as a fixed modification. Set protein N-terminal acetylation, oxidation of methionine, protein N-terminal methionine loss, protein N-terminal methionine loss and acetylation, and pyroglutamine formation as variable modifications. Allow a total of three variable modifications.

Data analysis

For persulfidation enrichment (Procedure D), the total peptide peak area for all cysteine-containing peptides for each protein is used to estimate the fold-change and is filtered to exclude proteins found in fewer than two replicates of either condition. For whole proteome analysis (Procedure E), peptides found in greater than or equal to two replicates are used to calculate the total area for each protein. The fractional abundance of each protein is calculated relative to the total lysate (protein area/total lysate area) and used to estimate the fold-change. In both datasets, to eliminate division by zero, proteins that are not identified in one of the two conditions are assigned a peptide area or fractional abundance that is equal to or one order of magnitude lower than the least abundant protein identified by the peak area.

While in the past we have analyzed protein persulfidation in terms of a sigma ratio, σ^R(Peng et al., 2017b), this approach is obviously unable to distinguish increased persulfidation status from increased protein abundance. We therefore normalize the change in persulfidation ((S/N)_SSH) to the change in protein abundance ((S/N)_Abund) as shown in Eq. 1. Those with normalized persulfidation changes that are greater than 4-fold are considered potential regulatory targets. This significance threshold can be altered depending on how many proteins are identified. Alternatively, these data can be visualized in a log-log plot of persulfidation vs. abundance change (Figure 1B). In this manner, we recommend filtering the proteins based on P-value for the change in persulfidation. Typically, a threshold of P < 0.05 is applied, but more stringent criteria can be used. We note that a P-value cannot be assigned to proteins identified only in either WT or Na₂S-treated cells and should not be eliminated from the analysis. In the log-log plot (Figure 1B), proteins that have significantly increased persulfidation status with either little-to-no change in protein abundance or decreased abundance are considered strong candidate regulatory targets of protein persulfidation, which requires validation using biochemical approaches using purified proteins.

Notes

While this protocol compares wild-type untreated cells with 0.2 mM Na₂S-treated cells, different amounts of Na₂S, incubation time (more or less than 30 min), or other sulfur donors, e.g., small molecule H₂S or intracellular persulfide donors (Walsh and Giedroc, 2020) can be used to investigate proteome persulfidation. We note, however, that the vast majority (≥85%) of cysteines detected as persulfidated in this workflow as a result of exogenous sulfide treatment are also persulfidated in untreated cells, consistent with significant “background” persulfidation of the proteome in exponentially growing bacterial cells (Peng et al., 2017b; Walsh et al., 2020).
The LC-MS analysis was performed by the Laboratory of Biological Mass Spectrometry at Indiana University.

Recipes

Na₂S stock
1. Weigh a small amount of Na₂S in a 1.5-ml screw cap vial and dissolve in sufficient water to prepare a 0.2 M solution.
2. Immediately prepare 100-μl aliquots in 1.5-ml tubes and store at -80°C.
3. Discard Na₂S stocks that are yellow in color as this is indicative of polysulfide impurities (Greiner et al., 2013; Luebke et al., 2014).
It is best to dissolve the Na₂S inside an anaerobic chamber using water that has been degassed and purged with argon. If an anaerobic chamber is not available, prepare the Na₂S stock immediately before use using degassed water.
PBS
8 g NaCl
0.2 g KCl
1.44 g Na₂HPO₄
0.24 g KH₂PO₄
Dissolve in 800 ml distilled H₂O
Adjust pH to 7.4
Sterilize by autoclaving
Lysis Buffer
25 mM MES
500 mM NaCl
pH 6.0
It is essential that these buffers be extensively degassed, purged with argon, and stored in an anaerobic chamber to minimize oxidation during cell lysis.
Sep-Pak Aqueous Buffer
5 ml 10% trifluoroacetic acid or formic acid
455 ml distilled H₂O
Sep-Pak Organic buffer
5 ml 10% trifluoroacetic acid or formic acid
250 ml acetonitrile
245 ml distilled H₂O

Acknowledgments

This work was supported by R35 GM118157 from the National Institutes of Health (NIH). B.J.C.W. was supported by a Kratz Fellowship and a Quantitative and Chemical Biology Graduate Training Fellowship provided by the NIH (T32 GM109825; T32 GM131994). This content does not necessarily represent the views of the NIH and is solely the responsibility of the authors.

Competing interests

The authors have no competing interests to declare.

References

Cuevasanta, E., Lange, M., Bonanata, J., Coitino, E. L., Ferrer-Sueta, G., Filipovic, M. R. and Alvarez, B. (2015). Reaction of Hydrogen Sulfide with Disulfide and Sulfenic Acid to Form the Strongly Nucleophilic Persulfide. J Biol Chem 290(45): 26866-26880.
Cuevasanta, E., Reyes, A. M., Zeida, A., Mastrogiovanni, M., De Armas, M. I., Radi, R., Alvarez, B. and Trujillo, M. (2019). Kinetics of formation and reactivity of the persulfide in the one-cysteine peroxiredoxin from Mycobacterium tuberculosis. J Biol Chem 294(37): 13593-13605.
Filipovic, M. R., Zivanovic, J., Alvarez, B. and Banerjee, R. (2018). Chemical Biology of H₂S Signaling through Persulfidation. Chem Rev 118(3): 1253-1337.
Fu, L., Liu, K., He, J., Tian, C., Yu, X. and Yang, J. (2020). Direct Proteomic Mapping of Cysteine Persulfidation. Antioxid Redox Signal 33(15): 1061-1076.
Gao, X. H., Krokowski, D., Guan, B. J., Bederman, I., Majumder, M., Parisien, M., Diatchenko, L., Kabil, O., Willard, B., Banerjee, R., Wang, B., Bebek, G., Evans, C. R., Fox, P. L., Gerson, S. L., Hoppel, C. L., Liu, M., Arvan, P. and Hatzoglou, M. (2015). Quantitative H₂S-mediated protein sulfhydration reveals metabolic reprogramming during the integrated stress response. Elife 4: e10067.
Greiner, R., Palinkas, Z., Basell, K., Becher, D., Antelmann, H., Nagy, P. and Dick, T. P. (2013). Polysulfides link H₂S to protein thiol oxidation. Antioxid Redox Signal 19(15): 1749-1765.
Ida, T., Sawa, T., Ihara, H., Tsuchiya, Y., Watanabe, Y., Kumagai, Y., Suematsu, M., Motohashi, H., Fujii, S., Matsunaga, T., Yamamoto, M., Ono, K., Devarie-Baez, N. O., Xian, M., Fukuto, J. M. and Akaike, T. (2014). Reactive cysteine persulfides and S-polythiolation regulate oxidative stress and redox signaling. Proc Natl Acad Sci U S A 111(21): 7606-7611.
Luebke, J. L., Shen, J., Bruce, K. E., Kehl-Fie, T. E., Peng, H., Skaar, E. P. and Giedroc, D. P. (2014). The CsoR-like sulfurtransferase repressor (CstR) is a persulfide sensor in Staphylococcus aureus. Mol Microbiol 94(6): 1343-1360.
Luhachack, L., Rasouly, A., Shamovsky, I. and Nudler, E. (2019). Transcription factor YcjW controls the emergency H₂S production in E. coli. Nat Commun 10(1): 2868.
Mironov, A., Seregina, T., Nagornykh, M., Luhachack, L. G., Korolkova, N., Lopes, L. E., Kotova, V., Zavilgelsky, G., Shakulov, R., Shatalin, K. and Nudler, E. (2017). Mechanism of H₂S-mediated protection against oxidative stress in Escherichiacoli. Proc Natl Acad Sci U S A 114(23): 6022-6027.
Park, C. M., Macinkovic, I., Filipovic, M. R. and Xian, M. (2015). Chapter Three - Use of the "tag-switch" method for the detection of protein S-sulfhydration. Methods Enzymol 555:39-56.
Peng, H., Shen, J., Edmonds, K. A., Luebke, J. L., Hickey, A. K., Palmer, L. D., Chang, F. J., Bruce, K. A., Kehl-Fie, T. E., Skaar, E. P. and Giedroc, D. P. (2017a). Sulfide Homeostasis and Nitroxyl Intersect via Formation of Reactive Sulfur Species in Staphylococcus aureus. mSphere 2(3): e00082-00017.
Peng, H., Zhang, Y., Palmer, L. D., Kehl-Fie, T. E., Skaar, E. P., Trinidad, J. C. and Giedroc, D. P. (2017b). Hydrogen Sulfide and Reactive Sulfur Species Impact Proteome S-Sulfhydration and Global Virulence Regulation in Staphylococcus aureus. ACS Infect Dis 3(10): 744-755.
Reisz, J. A., Bechtold, E., King, S. B., Poole, L. B. and Furdui, C. M. (2013). Thiol-blocking electrophiles interfere with labeling and detection of protein sulfenic acids. FEBS J 280(23): 6150-6161.
Saini, V., Chinta, K. C., Reddy, V. P., Glasgow, J. N., Stein, A., Lamprecht, D. A., Rahman, M. A., Mackenzie, J. S., Truebody, B. E., Adamson, J. H., Kunota, T. T. R., Bailey, S. M., Moellering, D. R., Lancaster, J. R., Jr. and Steyn, A. J. C. (2020). Hydrogen sulfide stimulates Mycobacterium tuberculosis respiration, growth and pathogenesis. Nat Commun 11(1): 557.
Shatalin, K., Shatalina, E., Mironov, A. and Nudler, E. (2011). H₂S: a universal defense against antibiotics in bacteria. Science 334(6058): 986-990.
Shen, J., Walsh, B. J. C., Flores-Mireles, A. L., Peng, H., Zhang, Y., Zhang, Y., Trinidad, J. C., Hultgren, S. J. and Giedroc, D. P. (2018). Hydrogen Sulfide Sensing through Reactive Sulfur Species (RSS) and Nitroxyl (HNO) in Enterococcusfaecalis. ACS Chem Biol 13(6): 1610-1620.
Shukla, P., Khodade, V. S., SharathChandra, M., Chauhan, P., Mishra, S., Siddaramappa, S., Pradeep, B. E., Singh, A. and Chakrapani, H. (2017). "On demand" redox buffering by H₂S contributes to antibiotic resistance revealed by a bacteria-specific H₂S donor.Chem Sci 8(7): 4967-4972.
Toliver-Kinsky, T., Cui, W., Toro, G., Lee, S. J., Shatalin, K., Nudler, E. and Szabo, C. (2019). H₂S, a Bacterial Defense Mechanism against the Host Immune Response. Infect Immun 87(1): e00272-00218.
Walsh, B. J. C. and Giedroc, D. P. (2020). H₂S and reactive sulfur signaling at the host-bacterial pathogen interface. J Biol Chem 295(38): 13150-13168.
Walsh, B. J. C., Wang, J., Edmonds, K. A., Palmer, L. D., Zhang, Y., Trinidad, J. C., Skaar, E. P. and Giedroc, D. P. (2020). The Response of Acinetobacter baumannii to Hydrogen Sulfide Reveals Two Independent Persulfide-Sensing Systems and a Connection to Biofilm Regulation. mBio 11(3): e01254-01220.
Zhang, D., Macinkovic, I., Devarie-Baez, N. O., Pan, J., Park, C. M., Carroll, K. S., Filipovic, M. R. and Xian, M. (2014). Detection of protein S-sulfhydration by a tag-switch technique. Angew Chem Int Ed Engl 53(2): 575-581.
Zhu, W., Smith, J. W. and Huang, C. M. (2010). Mass spectrometry-based label-free quantitative proteomics. J Biomed Biotechnol 2010: 840518.
Zivanovic, J., Kouroussis, E., Kohl, J. B., Adhikari, B., Bursac, B., Schott-Roux, S., Petrovic, D., Miljkovic, J. L., Thomas-Lopez, D., Jung, Y., Miler, M., Mitchell, S., Milosevic, V., Gomes, J. E., Benhar, M., Gonzalez-Zorn, B., Ivanovic-Burmazovic, I., Torregrossa, R., Mitchell, J. R., Whiteman, M., Schwarz, G., Snyder, S. H., Paul, B. D., Carroll, K. S. and Filipovic, M. R. (2019). Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration. Cell Metab 30(6): 1152-1170 e1113.