We checked the composition of Vogel-Bonner media and other media and salts in our publication (https://doi.org/10.21769/BioProtoc.2763) and found some mistakes in the units. New, correct units are as follows (in bold):

Recipes

Vogel-Bonner medium E (50x)
For Minimal agar (Recipe 9)

Ingredients	Per 500 ml
Warm distilled H₂O (40-50 °C)	335 ml
Magnesium sulfate (MgSO₄•7H₂O)	5 g
Citric acid monohydrate	50 g
Potassium phosphate, dibasic (anhydrous) (K₂HPO₄)	250 g
Sodium ammonium phosphate (NaNH₄HPO₄•4H₂O)	87.5 g

Salts are added to the warm water in a flask. Place the flask on a hot plate
After each salt dissolves entirely, transfer the solution into glass bottles and autoclave for 20 min at 121 °C
When the solution gets cool, cap the bottle tightly
Store the solution at 4 °C

Salt solution (1.65 M KCl + 0.4 M MgCl₂)
For S9 hepatic fraction
Ingredients
Per 250 ml
Potassium chloride (KCl)
30.75 g
Magnesium chloride (MgCl₂•6H₂O)
20.35 g
Distilled H₂O to final concentration of
250 ml
All the components are dissolved in water. The solution is autoclaved for 20 min, at 121 °C and then stored in refrigerator.
0.2 M sodium phosphate buffer, pH 7.4
For S9 hepatic fraction
Ingredients
Per 250 ml
0.2 M sodium dihydrogen phosphate (NaH₂PO₄•H₂O)
30 ml (6.9 g/250 ml)
0.2 M disodium hydrogen phosphate (Na₂HPO₄)
220 ml (7.1 g/250 ml)
Adjust pH to 7.4. Sterilize the buffer by autoclaving for 20 min at 121 °C

1 M glucose-6-phosphate
For S9 hepatic fraction
Ingredients
Per 5 ml
Glucose-6-phosphate (G-6-P)
1.41 g
Sterile distilled H₂O
5 ml
Glucose-6-phosphate is dissolved in the 5 ml distilled water and mixed by vortexing. Tubes are placed in an ice bath. The solution can be used for up to six months
Ampicillin solution (4 mg/ml)
Used in tests of ampicillin resistance
Master plates for R-factor strains
Ingredients
Per 500 ml
Ampicillin trihydrate
0.4 g
Sodium hydroxide (0.02 N)
50 ml
Ampicillin trihydrate is dissolved in the 50 ml of NaOH (0.02 N) and mixed by vortexing. Tubes are placed in an ice bath
Crystal violet solution (0.1%)
Used in tests for crystal violet sensitivity (to confirm rfa mutation)
Ingredients
Per 500 ml
Crystal violet
0.05 g
Distilled H₂O
50 ml
Minimal glucose plates
Used in Mutagenic bioassay
Ingredients
Per 500 ml
Agar
7.5 g
Distilled H₂O
465 ml
50x VB salts (Recipe 1)
10 ml
40% glucose
25 ml
Add agar in 465 ml of distilled water and autoclave for 20 min, at 121 °C. After cooling, add the salts and glucose gently
Histidine/Biotin plates (Master plates for non R-factor strains)
Used in tests for histidine requirement
Ingredients
Per 500 ml
Agar
7.5 g
Distilled H₂O
457 ml
50x VB salts
10 ml
40% glucose
25 ml
Sterile histidine (2 g per 400 ml H₂O)
5 ml
Sterile 0.5 mM biotin
3 ml
Dissolve agar in the given concentration in distilled water. Autoclave each solution separately for 20 min. After cooling of solution, add each salt gently

Ampicillin and tetracycline* plates
Master plates for the cultivation of strains containing the plasmids pKM101 and pAQ1*

Ingredients	Per 500 ml
Agar	7.5 g
Distilled H₂O	405 ml
50x VB salts	10 ml
40% glucose	25 ml
Sterile histidine (2 g per 400 ml H₂O)	5 ml
Sterile 0.5 mM biotin	3 ml
Sterile ampicillin solution (4 mg/ml 0.02 N NaOH)	1.58 ml
*Sterile tetracycline solution (4 mg/ml 0.02 N HCl)	0.125 ml

Dissolve agar in the given concentration in distilled water. Autoclave each solution separately for 20 min. After cooling of solution, add each salt gently
*Note: TA 102 is resistant to tetracycline. The shelf life of the plates is two weeks at 4 °C.

Nutrient agar plates
Used in tests for genotypes [Crystal violet sensitivity (rfa) and UV sensitivity (AuvrB)] and viability of bacteria
Ingredients
Per 500 ml
Nutrient agar
7.5 g
Distilled H₂O
500 ml
Dissolve agar in the given concentration in distilled water. Autoclave separately for 20 min. Pour the cooled solution into the Petri plates

References

Vijay, U., Gupta, S., Mathur, P., Suravajhala, P. and Bhatnagar, P. (2018). Microbial Mutagenicity Assay: Ames Test. Bio-protocol 8(6): e2763.