Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
小鼠骨髓来源的巨噬细胞(BMM’phi’)的分离培养
发布: 2012年02月05日第2卷第3期 DOI: 10.21769/BioProtoc.68 浏览次数: 69612
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Abstract
Bone marrow derived macrophages are a type of white blood cell that can be isolated from mammalian bone marrow. In this protocol, a method is described in which bone marrow cells are isolated from mouse leg bones (femur and tibia), and then differentiated to bone marrow-derived macrophages in approximately 10-day culture using L929-conditioned medium.
Keywords: Isolation (隔离)
Culture (培养)
Bone marrow-derived macrophages (骨髓来源的巨噬细胞)
Background
Materials and Reagents
- L929 cells
- RPMI 1640 medium (RPMI) (Life Technologies, InvitrogenTM, catalog number: 11875-093 )
- Fetal bovine serum (FBS) (Atlanta Biologicals, catalog number: S10350 )
- Stock penicillin/streptomycin (P/S) (Life Technologies, InvitrogenTM, catalog number: 15140-122 )
- DPBS (Life Technologies, InvitrogenTM, catalog number: 14190-250 )
- 75% ethanol
- Bone marrow growth medium (see Recipes)
- BMM’phi’ growth medium (see Recipes)
Equipment
- Cell culture incubator
- 0.22 μm filter
- 27 g needle and 1 ml syringe
- Scissors and forceps
- 15 ml cell culture dish
Procedure
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Chen, R. (2012). Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’). Bio-protocol 2(3): e68. DOI: 10.21769/BioProtoc.68.
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分类
免疫学 > 免疫细胞分离 > 维持和分化
细胞生物学 > 组织分析 > 组织分离
细胞生物学 > 细胞分离和培养 > 细胞分离
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