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Hi, I would like to know how many plates do you seed and at...

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Arturo Wilkins Sep 10, 2013
protocol Protocol: Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
Hi, I would like to know how many plates do you seed and at what cell density. Also, how do you dettach the macrophages once differentiated.
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yu liming Answered Jul 2, 2014

Nanjing Medical University

Hi! Ran, I used LPS to stimulate the mature BMDM growed in 150cm dishes for my CHIP analysis ,but I did find that gene of inflammation were inhibited strongly ,despite the mRNA was upregulated. RPMI 1640 with 10% FBS was used in my stimulation stage. Look forward for your reply.
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Ran Chen Author Answered Sep 10, 2013

Zhejiang JFK Biological Technology Co. Ltd.

Initially at 2 bones/10-cm dish and the cell density is around 10^4/ml.
We usually directly use cell lifters to scrape the the macrophages since the cells are very sticky to the plastic.
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