Culture and Detection of Mycobacterium tuberculosis (MTB) and Mycobacterium bovis (BCG)
结核分枝杆菌(MTB)和牛分枝杆菌(BCG)的培养和鉴定
发布: 2012年01月20日第2卷第1期 DOI: 10.21769/BioProtoc.49 浏览次数: 36326
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Abstract
Mycobacterium tuberculosis (MTB) is the bacterial pathogen responsible for tuberculosis, a human pulmonary infectious disease. Mycobacterium bovis (BCG) is the causative agent of tuberculosis in cattle, and is often used as the vaccine stain in humans. Specific recipes and methods for culture of MTB and BCG are described in this protocol.
Keywords: Mycobacterium tuberculosis (结核分枝杆菌)
Mycobacterium bovis BCG (卡介苗)
Culture (文化)
Materials and Reagents
- Middlebrook 7H9 broth base powder (Sigma-Aldrich, catalog number: M0178 )
- Middlebrook 7H11 agar base powder (Sigma-Aldrich, catalog number: M0428 )
- Middlebrook OADC growth supplement (Sigma-Aldrich, catalog number: M0678 )
- BD TB quick stain kit (BD Biosciences, catalog number: 212522 )
- General chemicals (Sigma-Aldrich)
- ADNaCl (see Recipes)
- 7H9 liquid medium (see Recipes)
- 7H11 agar plates (see Recipes)
Equipment
- T75 tissue culture flasks (Sigma-Aldrich, catalog number: Z707546 )
- Standard glass microscope slides
- Light microscope
- Bunsen burner or fixed flame source
- Standard sterile petri dishes
Procedure
文章信息
版权信息
© 2012 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Chen, R. (2012). Culture and Detection of Mycobacterium tuberculosis (MTB) and Mycobacterium bovis (BCG). Bio-protocol 2(1): e49. DOI: 10.21769/BioProtoc.49.
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分类
微生物学 > 微生物细胞生物学 > 细胞分离和培养
细胞生物学 > 细胞分离和培养 > 细胞生长
细胞生物学 > 细胞染色 > 其它化合物
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