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Question on protein concentration in the media:ADNaCl contains...

 Feb 22, 2013
protocol Protocol: Culture and Detection of Mycobacterium tuberculosis (MTB) and Mycobacterium bovis (BCG)
Question on protein concentration in the media:
ADNaCl contains 50 mg/mL BSA, dilute to 10% in the media to final 5 mg/mL BSA protein. Please confirm.

So MIC or any drug test in the media depends on the compound protein binding or free drug concentration?
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RAN CHEN Answered Feb 28, 2013

Stanford

The answer for the final concentration of BSA is yes. If the drug can be neutralized by BSA, of course you need to consider about the protein binding.
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