Isolation of Human PBMCs
人外周血单核细胞（PBMC）的分离
发布: 2013年02月05日第3卷第3期 DOI: 10.21769/BioProtoc.323 浏览次数: 97433
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参见作者原研究论文
The authors used this protocol in:
May 2012
Abstract
Peripheral blood mononuclear cells (PBMCs) are chiefly lymphocytes and monocytes. PBMCs are separated from the whole blood by a density gradient centrifugation method using Ficoll Histopaque.
Keywords: PBMC (外周血单个核细胞)
Human immune cells (人体免疫细胞)
In vitro Culture (体外培养)
Monocytes (单核细胞)
Lymphocytes (淋巴细胞)
Materials and Reagents
- Freshly collected heparinised blood
- Ficoll Histopaque (Sigma- Aldrich, catalog number: 10771 ; MP Biomedicals, catalog number: 091692254 )
- Sterile PBS or Dulbecco's modified eagle medium (catalog number: P-04-03590)
- Pencillin-streptomycin solution (Sigma- Aldrich, catalog number: P-4333 )
- Heparin vials (BD Biosciences, catalog number: 367886 )
- Dulbecco's modified eagle medium supplemented with 1% of Pencillin-streptomycin solution (see Recipes)
Equipment
- Centrifuge machine with swing-out bucket rotor (Eppendorf 5810 R )
- Sterile 15 ml centrifuge tube
- Auto pipettes and 1 ml tips
Procedure
文章信息
版权信息
© 2013 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Readers should cite both the Bio-protocol article and the original research article where this protocol was used:
- Panda, S. K. and Ravindran, B. (2013). Isolation of Human PBMCs. Bio-protocol 3(3): e323. DOI: 10.21769/BioProtoc.323.
- Panda, S. K., S. Kumar, N. C. Tupperwar, T. Vaidya, A. George, S. Rath, V. Bal and B. Ravindran (2012). Chitohexaose activates macrophages by alternate pathway through TLR4 and blocks endotoxemia. PLoS Pathog 8(5): e1002717.
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分类
免疫学 > 免疫细胞分离 > 淋巴细胞
细胞生物学 > 细胞分离和培养 > 细胞分离
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