Depends on the purpose. It is desirable to use heparin if PBMCs...

Balachandran Ravindran Oct 8, 2019

Protocol: Isolation of Human PBMCs

Depends on the purpose. It is desirable to use heparin if PBMCs are to be used for biological assays. Metabolic activity of the cells could be affected if EDTA is used. Centrifuging cells at 800g could lead to clumping of cells and cell viability could get compromised if the clumps are for Read more Read less

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