Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii
鲍氏不动杆菌的最小抑菌浓度（MIC）测定
发布: 2014年12月05日第4卷第23期 DOI: 10.21769/BioProtoc.1308 浏览次数: 47799
评审: Arsalan DaudiKanika GeraAnonymous reviewer(s)
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参见作者原研究论文
The authors used this protocol in:
May 2014
Abstract
Minimal inhibition concentration (MIC) is the lowest concentration of an antimicrobial agent that can inhibit the visible growth of a microorganism after overnight incubation. MIC determination is used as not only a diagnostic tool in treating bacterial infections for clinicians but also a research method in evaluating the efficacy of an antimicrobial. Multidrug resistance Acinetobacter baumannii (A. baumannii) has emerged in recent years. Accurate determination of resistance by MIC assay is important in coping with this superbug. Here we described a protocol for determining MIC for A. baumannii in hope of assisting researchers and physicians in confirming resistance of clinical isolates correctly.
Keywords: Acinetobacter baumannii (鲍氏不动杆菌)
Minimal Inhibitory concentration (最小抑菌浓度)
Drug resistance (耐药性)
Materials and Reagents
- A. baumannii (ATCC, catalog number: 17978 )
- Escherichia coli (E. coli) (ATCC, catalog number: 25922 )
- Mueller Hinton broth (Sigma-Aldrich, catalog number: 70192 )
- Tigecycline (Wyeth, catalog number. 0220620-09-7 )
- NaCl (MDBio, catalog number: 101-1647-14-5 )
- KCl (Sigma-Aldrich, catalog number: P1147 )
- Na2HPO4 (J.T.Baker®, catalog number: 3828-01 )
- KH2PO4 (J.T.Baker®, catalog number: 4921-07 )
- HCl (J.T.Baker®, catalog number: 9535-03 )
- Tryptone (Pronadisa, catalog number: 1612 )
- Yeast extract (Pronadisa, catalog number: 1702 )
- Cation-adjusted Mueller-Hinton broth (CAMHB) (see Recipes)
- PBS (1 L) (see Recipes)
- Lysogeny broth (LB) (see Recipes)
Equipment
- 50 ml polystyrene culture tubes (sterile)
- Spectrophotometer to measure absorbance of cell culture (OD600)
- 37 °C shaking and static incubators
- Multichannel pipette (volume ranges 10 μl-1,000 μl)
- 1.5 ml Eppendorf tube
- A centrifuge machine
- 1 ml cuvette
Procedure
文章信息
版权信息
© 2014 The Authors; exclusive licensee Bio-protocol LLC.
如何引用
Lin, M., Lin, Y. and Lan, C. (2014). Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii. Bio-protocol 4(23): e1308. DOI: 10.21769/BioProtoc.1308.
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分类
微生物学 > 抗微生物试验 > 抗细菌试验
细胞生物学 > 细胞分离和培养 > 细胞生长
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