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Please explain "the difference of measured and background OD600...

Ratcha Aunp Feb 1, 2017

Protocol: Minimal Inhibitory Concentration (MIC) Assay for Acinetobacter baumannii

Please explain "the difference of measured and background OD600 is less than 0.01."

What did you use for control and background?

Thanks

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Ming-Feng Lin Author Answered Feb 2, 2017

National Taiwan University Hospital Chu-Tung Branch

Answer: 900 µl CAMHB and 100 µl solutions of 10-time serial tested antibiotic concentration without adding bacteria was used as a negative control and its OD600 value was deemed as background value. The measured (no visible bacterial growh after the lowest concentration of antibiotics added) and background OD600 should be almost the same (difference less than 0.01).

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Chung-Yu Lan Author Answered Feb 2, 2017

Institute of Molecular and Cellular Biology, National Tsing Hua University

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