Fungi

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    Protocols in Current Issue
    Thermostability Measurement of an α-Glucosidase Using a Classical Activity-based Assay and a Novel Thermofluor Method
    Authors:  Karin Ernits, Katrin Viigand, Triinu Visnapuu, Kristina Põšnograjeva and Tiina Alamäe, date: 06/20/2017, view: 8602, Q&A: 0
    [Abstract] α-glucosidases (including maltases and isomaltases) are enzymes which release glucose from a set of α-glucosidic substrates. Their catalytic activity, substrate specificity and thermostability can be assayed using this trait. Thermostability of proteins can also be determined using a high-throughput differential scanning fluorometry method, also ...



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