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Thermostability Measurement of an α-Glucosidase Using a Classical Activity-based Assay and a Novel Thermofluor Method
Authors:
Karin Ernits
,
Katrin Viigand
,
Triinu Visnapuu
,
Kristina Põšnograjeva
and
Tiina Alamäe
,
date:
06/20/2017,
view:
8602,
Q&A:
0
[Abstract] α-glucosidases (including maltases and isomaltases) are enzymes which release glucose from a set of α-glucosidic substrates. Their catalytic activity, substrate specificity and thermostability can be assayed using this trait. Thermostability of proteins can also be determined using a high-throughput differential scanning fluorometry method, also ...
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