Promoter Orientation of Prokaryotic Phase-variable Genes by PCR
Authors: Stacey L. Bateman and
Patrick Seed,
date: 10/20/2012,
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[Abstract] One major mechanism of phase variable gene expression in prokaryotes is through inversion of the promoter element for a gene or operon. This protocol describes how to detect the promoter orientation of a phase-variable gene by PCR. This protocol, including primer design, is specific to detection of the promoter orientations of hyxR, a LuxR-like ...