Mammalia

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    Protocols in Current Issue
    Cortical Laminar Recording of Multi-unit Response to Distal Forelimb Electrical Stimulation in Rats
    Authors:  Charles-Francois V. Latchoumane, Rameen Forghani and Lohitash Karumbaiah, date: 11/20/2021, view: 1483, Q&A: 0
    [Abstract]

    Severe traumatic brain injury (sTBI) survivors experience permanent functional disabilities due to significant volume loss and the brain’s poor capacity to regenerate. Chondroitin sulfate glycosaminoglycans (CS-GAGs) are key regulators of growth factor signaling and neural stem cell homeostasis in the brain. In this protocol, we describe how

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    Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts
    [Abstract]

    The whole-cell patch-clamp method is a gold standard for single-cell analysis of electrical activity, cellular morphology, and gene expression. Prior to our discovery that patch-clamp pipettes could be cleaned and reused, experimental throughput and automation were limited by the need to replace pipettes manually after each experiment. This

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    Electrophysiological Properties of Neurons: Current-Clamp Recordings in Mouse Brain Slices and Firing-Pattern Analysis
    Authors:  Elina Nagaeva, Ivan Zubarev and Esa R. Korpi, date: 06/20/2021, view: 2624, Q&A: 0
    [Abstract]

    Characterization of an electrically active cell, such as a neuron, demands measurement of its electrical properties. Due to differences in gene activation, location, innervation patterns, and functions, the millions of neurons in the mammalian brain are tremendously diverse in their membrane characteristics and abilities to generate action

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    Transient Middle Cerebral Artery Occlusion with an Intraluminal Suture Enables Reproducible Induction of Ischemic Stroke in Mice
    [Abstract]

    Ischemic stroke is a leading cause of mortality and chronic disability worldwide, underscoring the need for reliable and accurate animal models to study this disease’s pathology, molecular mechanisms of injury, and treatment approaches. As most clinical strokes occur in regions supplied by the middle cerebral artery (MCA), several

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    Characterization of Hippocampal Adult-borne Granule Cells in a Transient Cerebral Ischemia Model
    Authors:  Mihai Ceanga, Madlen Guenther, Ina Ingrisch and Albrecht Kunze, date: 01/20/2021, view: 1367, Q&A: 0
    [Abstract]

    Long-term consequences of stroke significantly impair the quality of life in a growing population of stroke survivors. Hippocampal adult neurogenesis has been hypothesized to play a role in the pathophysiology of cognitive and neuropsychiatric long-term sequelae of stroke. Reliable animal models of stroke are paramount to understanding their

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    Preparation of Synaptoneurosomes to Study the Synapse in the Murine Cerebral Cortex
    Authors:  Ariel Diaz, Enrique Torre and Manuel Yepes, date: 01/20/2021, view: 2446, Q&A: 1
    [Abstract]

    The synapse is a complex structure where the transmission of information takes place. Synaptic dysfunction is one of the earliest pathophysiological events in several diseases, such as traumatic brain injury, cerebral ischemia, and neurodegenerative diseases. Thus, a methodology to study synaptic structure and function is crucial for the

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    Headpost Surgery for in vivo Electrophysiological Recording in the Mouse Inferior Colliculus during Locomotion
    Authors:  Yoonsun Yang and Gunsoo Kim, date: 12/05/2020, view: 1861, Q&A: 0
    [Abstract]

    The inferior colliculus (IC) is a critical midbrain integration center for auditory and non-auditory information. Although much is known about the response properties of the IC neurons to auditory stimuli, how the IC neural circuits function during movement such as locomotion remains poorly understood. Mice offer a valuable model in this respect,

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    Preparing Viable Hippocampal Slices from Adult Mice for the Study of Sharp Wave-ripples
    Authors:  Linhua Liu, Xiaojing Zhou and Jian-young Wu, date: 10/05/2020, view: 2268, Q&A: 0
    [Abstract] We describe a protocol for preparing acute brain slices which can produce robust hippocampal sharp wave-ripples (SWRs) in vitro. The protocol is optimized for its simplicity and reliability for the preparation of solutions, slicing, and recovery incubation. Most slices in almost every mouse prepared though the protocol expressed vigorous ...
    Hippocampal Unicellular Recordings and Hippocampal-dependent Innate Behaviors in an Adolescent Mouse Model of Alzheimer’s disease
    [Abstract] Transgenic mice have been used to make valuable contributions to the field of neuroscience and model neurological diseases. The simultaneous functional analysis of hippocampal cell activity combined with hippocampal dependent innate task evaluations provides a reliable experimental approach to detect fine changes during early phases of ...
    Induction of Temporal Lobe Epilepsy in Mice with Pilocarpine
    Authors:  Muhammad Nauman Arshad and Janice R Naegele, date: 02/20/2020, view: 4226, Q&A: 0
    [Abstract] In the pilocarpine model of temporal lobe epilepsy (TLE) in rodents, systemic injections of pilocarpine induce continuous, prolonged limbic seizures, a condition termed “Status Epilepticus” (SE). With appropriate doses, many inbred strains of mice show behavioral seizures within an hour after pilocarpine is injected. With the behavioral scoring ...



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