Mammalia

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    Protocols in Current Issue
    In situ Hybridization (ISH) and Quantum Dots (QD) of miRNAs
    Authors:  Sajni Josson, Murali Gururajan and Leland W.K. Chung, date: 02/20/2017, view: 9196, Q&A: 0
    [Abstract] miRNA are short non-coding RNA which inhibit translation of mRNA. miRNA regulate several cellular processes. Certain miRNA are known to induce oncogenesis. miRNA can be measured by real-time PCR and be imaged using a combination of in situ hybridization (ISH) and quantum dots (QD). The advantage of using quantum dots is that several miRNA ...
    miRNA Characterization from the Extracellular Vesicles
    Authors:  Sajni Josson, Murali Gururajan and Leland W.K. Chung, date: 02/20/2017, view: 6710, Q&A: 0
    [Abstract] Cancer cells and cancer associated stromal cells co-evolve secrete extracellular vesicles to the surrounding regions and regulate several processes involved in cancer metastasis. miRNAs have been known to be mediators of cancer progression and metastasis. miRNAs consist of short noncoding RNA. miRNAs are stable in extracellular fluids such as ...



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