Protocols in Current Issue
    EdU labeling of Trypanosome Cells and Their Kinetoplast DNA (kDNA)
    Author:  Jianyang Wang, date: 06/20/2013, view: 9304, Q&A: 0
    [Abstract] Trypanosome mitochondrial genome, known as Kinetoplast DNA (kDNA), is a massive network of interlocked DNA rings. The studies of kDNA replication and architecture are of major significance since kDNA is a valid drug target. However, DNA in procyclic trypanosomes can not be labeled with tracer concentrations of 3[H]-thymidine, possibly ...

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