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Measuring Auxin Transport Capacity in Seedling Roots of
Medicago truncatula
Authors:
Jason Liang Pin Ng
and
Ulrike Mathesius
,
date:
06/20/2016,
view:
8363,
Q&A:
0
[Abstract] Measurement of auxin transport capacity provides quantitative data on the physiological machinery involved in auxin transport within plants. This technique is easy to perform and gives quick results. Radiolabelled auxin (indole-3-acetic-acid) is fed into the roots of
Medicago truncatula
via an agar block. The resulting radioactivity from ...
Quantifying Auxin Metabolites in Young Root Tissue of
Medicago truncatula
by Liquid Chromatography Electrospray-ionisation Quadrupole Time-of-flight (LC-ESI-QTOF) Tandem Mass Spectrometry
Authors:
Jason Liang Pin Ng
,
Thy T. Truong
,
Charles H. Hocart
and
Ulrike Mathesius
,
date:
06/20/2016,
view:
8778,
Q&A:
0
[Abstract] Auxins represent a major group of phytohormones controlling plant development. The spatio-temporal regulation of auxin gradients is essential for the initiation, growth and correct development of plant organs. Because auxins and their metabolites occur at trace levels in plant tissue, experiments requiring identification plus their selective and ...
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