Mammalia

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    Protocols in Current Issue
    Antisense Oligo Pulldown of Circular RNA for Downstream Analysis
    Authors:  Debojyoti Das, Aniruddha Das and Amaresh C. Panda, date: 07/20/2021, view: 1860, Q&A: 0
    [Abstract]

    Circular RNAs (circRNAs) are a large family of noncoding RNA molecules that have emerged as novel regulators of gene expression by sequestering microRNAs (miRNAs) and RNA-binding proteins (RBPs). Several computational tools have been developed to predict circRNA interaction with target miRNAs and RBPs with a view to studying their potential effect

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    Pancreatic Acinar Cell Preparation for Oxygen Consumption and Lactate Production Analysis
    Authors:  Jane A. Armstrong, Robert Sutton and David N. Criddle, date: 05/20/2020, view: 2683, Q&A: 0
    [Abstract] Mitochondrial dysfunction is a principal feature of acute pancreatitis (AP) although the underlying mechanisms are still unclear. AP precipitants induce Ca2+-dependent formation of the mitochondrial permeability transition pore (MPTP) in pancreatic acinar cells (PACs), leading to ATP depletion and necrosis. Evaluations of mitochondrial ...
    Murine Pancreatic Islets Transplantation under the Kidney Capsule
    [Abstract] Type 1 diabetes (T1D) is an autoimmune disease caused by the lack of insulin-producing pancreatic beta cells leading to systemic hyperglycemia. Pancreatic islet transplantation is a valid therapeutic approach to restore insulin loss and to promote adequate glycemic control. Pancreatic islet transplantation in mice is an optimal preclinical model ...
    Isolation and Culture of the Islets of Langerhans from Mouse Pancreas
    Authors:  Kate L Graham, Stacey Fynch, Evan G Pappas, Christina Tan, Thomas WH Kay and Helen E Thomas, date: 06/20/2016, view: 22014, Q&A: 1
    [Abstract] The islets of Langerhans are clusters of endocrine cells located within the pancreas. Insulin-producing beta cells are the major cell type within islets, with glucagon-producing alpha cells and somatostatin-producing delta cells the other major cell types. The beta cells are the target of immune-mediated destruction in type 1 diabetes (Graham et ...



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