Prokaryotes

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    Protocols in Current Issue
    Purification of Bacterial RNA from Infected Macrophages
    Authors:  Lior Lobel, Nadejda Sigal, Anna Pasechnek and Anat A. Herskovits, date: 11/20/2015, view: 9349, Q&A: 0
    [Abstract] Studying the transcriptome of bacterial pathogens during infection is a very informative and effective tool for discovering genes that contribute to successful infection. However, isolating bacterial RNA from infected cells or tissues is a challenging process due to the much higher amounts of host RNA in the lysates of infected cells. We have ...



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