Plants

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    Protocols in Current Issue
    A Novel Method to Map Small RNAs with High Resolution
    Authors:  Kun Huang, Feray Demirci, Blake C. Meyers and Jeffrey L. Caplan, date: 08/20/2021, view: 1797, Q&A: 0
    [Abstract]

    Analyzing cellular structures and the relative location of molecules is essential for addressing biological questions. Super-resolution microscopy techniques that bypass the light diffraction limit have become increasingly popular to study cellular molecule dynamics in situ. However, the application of super-resolution imaging techniques to detect

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    Purification and Cryo-electron Microscopy Analysis of Plant Mitochondrial Ribosomes
    Authors:  Florent Waltz, Philippe Giegé and Yaser Hashem, date: 08/05/2021, view: 1583, Q&A: 0
    [Abstract]

    Plants make up by far the largest part of biomass on Earth. They are the primary source of food and the basis of most drugs used for medicinal purposes. Similarly to all eukaryotes, plant cells also use mitochondria for energy production. Among mitochondrial gene expression processes, translation is the least understood; although, recent advances

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    Production of Guide RNAs in vitro and in vivo for CRISPR Using Ribozymes and RNA Polymerase II Promoters
    Authors:  Tao Zhang, Yangbin Gao, Rongchen Wang and Yunde Zhao, date: 02/20/2017, view: 15140, Q&A: 0
    [Abstract] CRISPR/Cas9-mediated genome editing relies on a guide RNA (gRNA) molecule to generate sequence-specific DNA cleavage, which is a prerequisite for gene editing. Here we establish a method that enables production of gRNAs from any promoters, in any organisms, and in vitro (Gao and Zhao, 2014). This method also makes it feasible to conduct ...
    Dot Blot Analysis of N6-methyladenosine RNA Modification Levels
    Authors:  Lisha Shen, Zhe Liang and Hao Yu, date: 01/05/2017, view: 24142, Q&A: 3
    [Abstract] N6-methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). The total amount of m6A can be detected by several methods, such as dot blot analysis using specific m6A antibodies and quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Fu et al ...
    Computational Identification of MicroRNA-targeted Nucleotide-binding Site-leucine-rich Repeat Genes in Plants
    Authors:  Zhu-Qing Shao, Yan-Mei Zhang, Bin Wang and Jian-Qun Chen, date: 11/05/2015, view: 9059, Q&A: 0
    [Abstract] Plant genomes harbor dozens to hundreds of nucleotide-binding site-leucine-rich repeat (NBS-LRR, NBS for short) type disease resistance genes (Shao et al., 2014; Zhang et al., 2015). Proper regulation of these genes is important for normal growth of plants by reducing unnecessary fitness costs in the absence of pathogen ...
    Circular RT-PCR Assay Using Arabidopsis Samples
    Authors:  Runlai Hang, Xian Deng, Chunyan Liu, Beixin Mo and Xiaofeng Cao, date: 07/20/2015, view: 14873, Q&A: 0
    [Abstract] Post-transcriptional processing is critical for RNA biogenesis, in which conventional functional RNA transcripts are generated, such as messenger RNAs (mRNAs), transfer RNAs (tRNAs) and ribosomal RNAs (rRNAs) for translation as well as emerging non-coding RNAs with known or unknown regulatory functions. To determine the precise termini of an RNA ...



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