Protocols in Current Issue
    Multitarget Immunohistochemistry for Confocal and Super-resolution Imaging of Plant Cell Wall Polysaccharides
    Authors:  Kalina T. Haas, Methieu Rivière, Raymond Wightman and Alexis Peaucelle, date: 10/05/2020, view: 3655, Q&A: 0
    [Abstract] The plant cell wall (PCW) is a pecto-cellulosic extracellular matrix that envelopes the plant cell. By integrating extra-and intra-cellular cues, PCW mediates a plethora of essential physiological functions. Notably, it permits controlled and oriented tissue growth by tuning its local mechano-chemical properties. To refine our knowledge of these ...
    Single-run HPLC Quantification of Plant Cell Wall Monosaccharides
    [Abstract] The plant cell wall is a complex network of polysaccharides and proteins that provides strength and structural integrity to plant cells, as well as playing a vital role in growth, development, and defense response. Cell wall polysaccharides can be broadly grouped into three categories: cellulose, pectins, and hemicelluloses. Dynamic interactions ...
    Detection of Hydroxyproline O-galactoside by LC/MS
    Authors:  Mari Ogawa-Ohnishi and Yoshikatsu Matsubayashi, date: 01/20/2016, view: 5836, Q&A: 0
    [Abstract] Hydroxyproline (Hyp) O-galactosylation is a plant-specific post-translational modification found in extracellular glycoproteins such as arabinogalactan proteins (AGPs). Hyp O-galactosylation is mediated by Hyp O-galactosyltransferase (HPGT) that catalyzes the transfer of a D-galactopyranosyl residue to the hydroxyl group ...
    Saccharification Protocol for Small-scale Lignocellulosic Biomass Samples to Test Processing of Cellulose into Glucose
    Authors:  Rebecca Van Acker, Ruben Vanholme, Kathleen Piens and Wout Boerjan, date: 01/05/2016, view: 10406, Q&A: 0
    [Abstract] Second generation biofuels are derived from inedible lignocellulosic biomass of food and non-food crops. Lignocellulosic biomass is mainly composed of cell walls that contain a large proportion of cellulosic and hemicellulosic polysaccharides. An interesting route to generate biofuels and bio-based materials is via enzymatic hydrolysis of cell ...
    Analysis of in vivo Cellulose Biosynthesis in Arabidopsis Cells by Spinning Disk Confocal Microscopy
    Authors:  Tamara Vellosillo, Trevor Yeats and Nadav Sorek, date: 10/05/2015, view: 8035, Q&A: 0
    [Abstract] Cellulose is a main component of plant cell walls. Tools to analyze cellulose mainly rely on analytical chemistry, which yields information about cellulose amounts and structure, but cannot be applied to intact tissues. Moreover, these methods measure total cellulose and cannot be used to assay cellulose synthesis per se. Live cell ...
    [14C] Glucose Cell Wall Incorporation Assay for the Estimation of Cellulose Biosynthesis
    Authors:  Chad Brabham, Jozsef Stork and Seth Debolt, date: 09/20/2015, view: 6264, Q&A: 0
    [Abstract] Cellulose is synthesized by Cellulose Synthase A proteins at the plasma membrane using the substrate UDP glucose. Herein, we provide a detailed method for measuring the incorporation of radiolabeled glucose into the cellulose fraction of the cell wall. In this method Arabidopsis seedlings are treated for 2 h with a cellulose biosynthesis ...
    Staining of Callose Depositions in Root and Leaf Tissues
    Authors:  Sebastian T. Schenk and Adam Schikora, date: 03/20/2015, view: 23998, Q&A: 0
    [Abstract] The plant cell wall is a physical barrier, which fulfills a plethora of functions, for example it can efficiently prevent pathogen’s entry into the cell. In addition, its changing composition contributes to plants inducible defense mechanisms. This layer of defense includes pathogen perception and is followed by the activation of defense responses ...

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