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Protocol for Enrichment of the Membrane Proteome of Mature Tomato Pollen
Authors:
Puneet Paul
,
Palak Chaturvedi
,
Anida Mesihovic
,
Arindam Ghatak
,
Wolfram Weckwerth
and
Enrico Schleiff
,
date:
06/05/2017,
view:
6819,
Q&A:
0
[Abstract] We established and elaborated on a method to enrich the membrane proteome of mature pollen from economically relevant crop using the example of
Solanum lycopersicum
(tomato). To isolate the pollen protein fraction enriched in membrane proteins, a high salt concentration (750 mM of sodium chloride) was used. The membrane protein-enriched ...
Measurement of Cellular Redox in Pollen with Redox-Sensitive GFP (roGFP) Using Live Cell Imaging
Authors:
Wei-Jie Huang
and
Wei-Hua Tang
,
date:
03/05/2015,
view:
9371,
Q&A:
0
[Abstract] Redox homeostasis is a fundamental property of living cells and responds actively to both cellular metabolism and external stimulus. The development of redox-sensitive GFP (roGFP) enables dynamic monitoring of changes in cellular redox poise (Hanson
et al.
, 2014). When excited alternatively at 405 nm and 488 nm, these probes exhibit ...
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