Prokaryotes

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    In vitro DNA Polymerization Activity Assay Using Cell-free Extracts
    Authors:  Anurag K. Sinha and Malay K. Ray, date: 08/20/2014, view: 7976, Q&A: 0
    [Abstract] This protocol has been designed to measure the in-vitro DNA polymerization activity in crude cell extracts of the Antarctic bacterium Pseudomonas syrinagae Lz4W. This bacterium can grow at 4 °C with optimum growth rate at 22 °C. The slow growth rate of the bacterium observed at low temperature (4 °C) compared to higher ...



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