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Determination of the Secondary Structure of an RNA fragment in Solution: Selective 2`-Hydroxyl Acylation Analyzed by Primer Extension Assay (SHAPE)
Authors:
Manuel Miras
,
Raquel N. Sempere
,
Jelena J. Kraft
,
W. Allen Miller
,
Miguel A. Aranda
and
Veronica Truniger
,
date:
01/20/2015,
view:
12195,
Q&A:
0
[Abstract] This protocol describes the methodology for the determination of the secondary structure of an RNA fragment in solution using Selective 2´-Hydroxyl Acylation analyzed by Primer Extension, abbreviation SHAPE. It consists in the very fast chemical modification of flexible and therefore possibly single-stranded nucleotides in a sequence-independent ...
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