Protocols in Current Issue
    Detection of Mitogen-activated Protein Kinase (MAPK) Activation upon Exogenous Chemical Application in Arabidopsis Protoplasts
    Authors:  Arsheed Hussain Sheikh and Alok Krishna Sinha, date: 09/20/2015, view: 10439, Q&A: 0
    [Abstract] The mitogen activated protein kinase cascade is a highly conserved signal pathway in plants. The exogenous chemicals, like hormones, can trigger a series of signalling cascades, including MAPK pathway, to modulate the plant physiology. Upon activation, some MAPKs are phosphorylated. It is important to develop methods that can detect changes in the ...
    Measurement of the Number of Peroxisomes
    Authors:  Michitaro Shibata, Kazusato Oikawa, Shoji Mano and Mikio Nishimura, date: 11/05/2014, view: 9156, Q&A: 0
    [Abstract] This is the detailed protocol for the measurement of the number of peroxisomes described by Shibata et al. (2013). It is difficult to count the number of organelles in a cell because of the thickness of plant leaves. To overcome this challenge, protoplasts were isolated from leaves, and the number of peroxisomes per protoplast was ...
    Protoplast Preparation and Determination of Cell Death
    Author:  Antoine Danon, date: 06/20/2014, view: 16805, Q&A: 3
    [Abstract] The protoplasts assay constitutes a powerful tool that allows an easy uptake of active agents and a precise quantification of cell death induction in different populations. Our study showed that the basal level of cell death in our controls is low and stable throughout the length of our experiments (Danon et al., 2005; Pineau et al. ...

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