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Fluorescence-linked Antigen Quantification (FLAQ) Assay for Fast Quantification of HIV-1 p24
Gag
Authors:
Marianne Gesner
,
Mekhala Maiti
,
Robert Grant
and
Marielle Cavrois
,
date:
12/20/2014,
view:
10093,
Q&A:
1
[Abstract] The fluorescence-linked antigen quantification (FLAQ) assay allows a fast quantification of HIV-1 p24
Gag
antigen. Viral supernatant are lysed and incubated with polystyrene microspheres coated with polyclonal antibodies against HIV-1 p24
Gag
and detector antibodies conjugated to fluorochromes (Figure 1). After washes, the ...
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