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    Protocols in Current Issue
    Fluorescence-linked Antigen Quantification (FLAQ) Assay for Fast Quantification of HIV-1 p24Gag
    Authors:  Marianne Gesner, Mekhala Maiti, Robert Grant and Marielle Cavrois, date: 12/20/2014, view: 10093, Q&A: 1
    [Abstract] The fluorescence-linked antigen quantification (FLAQ) assay allows a fast quantification of HIV-1 p24Gag antigen. Viral supernatant are lysed and incubated with polystyrene microspheres coated with polyclonal antibodies against HIV-1 p24Gag and detector antibodies conjugated to fluorochromes (Figure 1). After washes, the ...



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