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    Protocols in Current Issue
    TUNEL Labeling to Detect Double-stranded DNA Breaks in Caenorhabditis elegans Gonads
    Authors:  Peter A. Kropp, Kyle Rhodehouse and Andy Golden, date: 03/20/2022, view: 408, Q&A: 0
    [Abstract]

    Analysis of DNA double strand breaks (DSBs) is important for understanding dyshomeostasis within the nucleus, impaired DNA repair mechanisms, and cell death. In the C. elegans germline, DSBs are important indicators of all three above-mentioned conditions. Although multiple methods exist to assess apoptosis in the germline of C. elegans, direct

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    Microirradiation for Precise, Double-strand Break Induction in vivo in Caenorhabditis elegans
    Authors:  Kailey E. Harrell, Emily Koury and Sarit Smolikove, date: 12/20/2018, view: 3817, Q&A: 0
    [Abstract] DNA double-strand breaks (DSBs) are toxic lesions that every cell must accurately repair in order to survive. The repair of DSBs is an integral part of a cell life cycle and can lead to lethality if repaired incorrectly. Laser microirradiation is an established technique which has been used in yeast, mammalian cell culture, and Drosophila ...
    Generation of Caenorhabditis elegans Transgenic Animals by DNA Microinjection
    Authors:  Matthias Rieckher and Nektarios Tavernarakis, date: 10/05/2017, view: 11440, Q&A: 0
    [Abstract] Microinjection is the most frequently used tool for genetic transformation of the nematode Caenorhabditis elegans, facilitating the transgenic expression of genes, genome editing by the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, or transcription of dsRNA for RNA intereference (RNAi). Exogenous DNA is ...
    PCR-RFLP Genotyping of Point Mutations in Caenorhabditis elegans
    Author:  Peichuan Zhang, date: 03/20/2012, view: 24104, Q&A: 4
    [Abstract] This protocol describes the basic principle of PCR/restriction digest genotyping of point mutations in worms, based on the principle of Restriction Fragment Length Polymorphism (RFLP) analysis. This type of genotyping is, particularly, useful when phenotypic analysis of animals carrying point mutations is difficult (e.g., in a complex ...



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