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Single Genome Sequencing of Expressed and Proviral HIV-1 Envelope Glycoprotein 120 (
gp120
) and
nef
Genes
Authors:
David J. Nolan
,
Susanna L. Lamers
,
Rebecca Rose
,
James J. Dollar
,
Marco Salemi
and
Michael S. McGrath
,
date:
06/20/2017,
view:
8083,
Q&A:
0
[Abstract] The current study provides detailed protocols utilized to amplify the complete HIV-1
gp120
and
nef
genes from single copies of expressed or integrated HIV present in fresh-frozen autopsy tissues of patients who died while on combined antiretroviral therapy (cART) with no detectable plasma viral load (pVL) at death (Lamers
et al
...
Quantification of Total and 2-LTR (Long terminal repeat) HIV DNA, HIV RNA and Herpesvirus DNA in PBMCs
Authors:
Marta Massanella
,
Sara Gianella
,
Steven M. Lada
,
Douglas D. Richman
and
Matthew C. Strain
,
date:
06/05/2015,
view:
11978,
Q&A:
0
[Abstract] Almost all individuals infected with human immunodeficiency virus (HIV) are also infected with cytomegalovirus (CMV) and Epstein Barr virus (EBV). The aims of our studies have included characterizing and measuring the latent HIV reservoir and understanding the association between asymptomatic replication of CMV (and other herpesvirus, including ...
Cell Fractionation and Quantitative Analysis of HIV-1 Reverse Transcription in Target Cells
Authors:
Vaibhav B Shah
and
Christopher Aiken
,
date:
10/20/2013,
view:
9451,
Q&A:
0
[Abstract] This is a protocol to detect HIV-1 reverse transcription products in cytoplasmic and nuclear fractions of cells infected with VSV-G-pseudotyped envelope-defective HIV-1. This protocol can also be extended to HIV-1 with regular envelope.
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