Virus-based MicroRNA Silencing
Authors: Jinping Zhao and
Yule Liu,
date: 01/20/2016,
view: 9436,
Q&A: 0
[Abstract] Virus-based microRNA silencing (VbMS) is a viable and prompt method to screen and characterize the function of microRNAs (miRNAs) in plants. The Tobacco rattle virus (TRV)-based VbMS method was originally developed by the Yule Liu's group (Sha et al., 2014) using miRNA target mimic (TM) methodology. Here, we describe the ...
VIGS Assays
Authors: Haili Zhang and
Yule Liu,
date: 03/05/2014,
view: 23911,
Q&A: 0
[Abstract] Virus-induced gene silencing (VIGS) is a powerful method to study gene function in plants. Tobacco rattle virus (TRV)-based VIGS vector is the most efficient VIGS vector so far. This method was originally developed by the Dinesh-Kumar's group (Liu et al., 2002) . Here, we describe a rapid and high efficient TRV-based VIGS method for ...