Protocols in Current Issue
    Preparation of cDNA Library for dRNA-seq
    Authors:  Feng Li and Barbara Baker, date: 12/05/2012, view: 14332, Q&A: 0
    [Abstract] microRNAs (miRNAs) are ubiquitous regulators of gene expression in eukaryotic organisms, which guide Argonaute proteins (AGO) to cleave target mRNA or inhibit its translation based on sequence complementarity. In plants, miRNA directed cleavage occurs on the target mRNA at about 10 to 11 nucleotide (nt) up stream to the site where the 5’ end of ...

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