Other Vertebrates

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    Protocols in Current Issue
    Expression and Analysis of Flow-regulated Ion Channels in Xenopus Oocytes
    Authors:  Shujie Shi and Marcelo D. Carattino, date: 04/20/2017, view: 8342, Q&A: 0
    [Abstract] Mechanically-gated ion channels play key roles in mechanotransduction, a process that translates physical forces into biological signals. Epithelial and endothelial cells are exposed to laminar shear stress (LSS), a tangential force exerted by flowing fluids against the wall of vessels and epithelia. The protocol outlined herein has been used to ...
    Acquisition of Leftward Flow in Xenopus laevis
    Authors:  Thomas Thumberger and Martin Blum, date: 12/05/2013, view: 8669, Q&A: 0
    [Abstract] In Xenopus, the left-right axis is established following an extracellular vectorial leftward flow driven by monocilia at the gastrocoel roof plate (GRP) during late gastrulation / neurulation (Schweickert et al., 2007). As the GRP lies inside the developing archenteron, imaging of flow is challenging. Here we present the detailed ...



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