Protocols in Current Issue
    In vivo Use of Dextran-based Anterograde Cortical Tracers to Assess the Integrity of the Cortical Spinal Tract
    Authors:  Anna M. Wiersma and Ian R. Winship, date: 05/20/2018, view: 5680, Q&A: 0
    [Abstract] When injected into the motor cortex of rats, anterograde tracers label fibers of the associated descending corticospinal tract (CST) that originate from pyramidal neurons in the tracer-injected cortex. These fibers can be assessed at the level of the spinal cord to determine the integrity of the descending CST and the spatial distribution of axons ...
    Sacral Spinal Cord Transection and Isolated Sacral Cord Preparation to Study Chronic Spinal Cord Injury in Adult Mice
    Authors:  Carmelo Bellardita, Maite Marcantoni, Peter Löw and Ole Kiehn, date: 04/05/2018, view: 6056, Q&A: 0
    [Abstract] Spinal cord injury (SCI) is characterized by multiple sensory/motor impairments that arise from different underlying neural mechanisms. Linking specific sensory/motor impairments to neural mechanism is limited by a lack of direct experimental access to these neural circuits. Here, we describe an experimental model which addresses this shortcoming. ...
    Contusion Spinal Cord Injury Rat Model
    Authors:  Chuan-Wen Chiu, Henrich Cheng and Shie-Liang Hsieh, date: 06/20/2017, view: 10091, Q&A: 0
    [Abstract] Spinal cord injury (SCI) can lead to severe disability, paralysis, neurological deficits and even death. In humans, most spinal cord injuries are caused by transient compression or contusion of the spinal cord associated with motor vehicle accidents. Animal models of contusion mimic the typical SCI’s found in humans and these models are key to the ...
    Peripheral Nerve Injury: a Mouse Model of Neuropathic Pain
    Authors:  Takahiro Masuda, Yuta Kohro, Kazuhide Inoue and Makoto Tsuda, date: 05/05/2017, view: 10333, Q&A: 0
    [Abstract] Neuropathic pain is one of the highly debilitating chronic pain conditions, for which, currently, there is no therapeutic treatment. In order to reveal the underlying mechanism for neuropathic pain, various animal models have been established (Burma et al., 2016). This protocol describes how to prepare spinal nerve injury model (Kim and ...
    In vitro Brainstem-spinal Cord Preparation from Newborn Rat
    Authors:  Jean-Patrick Le Gal, Angelo Nicolosi, Laurent Juvin and Didier Morin, date: 11/20/2016, view: 7864, Q&A: 0
    [Abstract] The brainstem-spinal cord preparation of newborn rat contains neural networks able to produce motor output in absence of sensory feedback. These neural structures, commonly called central pattern generators (CPGs), are involved in many vital functions such as respiration (Morin and Viala, 2002; Giraudin et al., 2008) or locomotion (Juvin ...
    Morphological Quantification of Nuclei and Mitochondria in Serial Block-face Scanning Electron Microscopy Images
    Authors:  Haiyan Lu, Nobuhiko Ohno and Richard M. Ransohoff, date: 09/20/2015, view: 8127, Q&A: 0
    [Abstract] Serial Block-face Scanning Electron Microscopy (SBF-SEM or 3D-EM) is a powerful tool to study biological structure in ultrastructural level. Quantification of cellular ultrastructure is useful to providing biological information. This technique requires not only high quality of tissue fixation and ideal sample embedding to preserve structures, ...
    Mitochondrial Isolation and Purification from Mouse Spinal Cord
    Authors:  Philippe A. Parone, Sandrine Da Cruz and Don W. Cleveland, date: 11/05/2013, view: 10696, Q&A: 0
    [Abstract] Mitochondria are eukaryotic organelles that play a crucial role in several cellular processes, including energy production, β-oxidation of fatty acids and regulation of calcium homeostasis. In the last 20 years there has been a hightened interest in the study of mitochondria following the discoveries that mitochondria are central to the process of ...
    Rat Model of Chronic Midthoracic Lateral Hemisection
    Author:  Victor Arvanian, date: 09/20/2013, view: 9960, Q&A: 0
    [Abstract] Although most spinal cord injuries (SCI) are anatomically incomplete, only limited functional recovery has been observed in people and rats with partial lesions. To address why surviving fibers cannot mediate more complete recovery, it is important to evaluate the physiological and anatomical status of spared fibers. These experiments require use ...
    Generation of Mouse Spinal Cord Injury
    Authors:  Oneil G. Bhalala, Liuliu Pan, Hilary North, Tammy McGuire and John A. Kessler, date: 09/05/2013, view: 18631, Q&A: 1
    [Abstract] Spinal cord injury (SCI) is a debilitating injury with significant morbidity and mortalitiy. Understanding the pathogensis of and developing treatments for SCI requires robust animal models. Here we describe a method for generating an efficient and reproducible contusion model of SCI in adult mice.

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