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Protocol for Isolation of Cardiomyocyte from Adult Mouse and Rat
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PEG-mediated, Stable, Nuclear and Chloroplast Transformation of
Cyanidioschizon merolae
Authors:
Maksymilian Zienkiewicz
,
Tomasz Krupnik
,
Anna Drożak
and
Kinga Kania
,
date:
09/05/2019,
view:
3086,
Q&A:
0
[Abstract] The ability to achieve nuclear or chloroplast transformation in plants has been a long standing goal, especially in microalgae research. Over past years there has been only little success, but transient and stable nuclear transformation has been achieved in multiple species. Our newly developed method allows for relatively simple transformation of
...
Preparation of Knockdown Transformants of Unicellular Charophycean Alga,
Closterium peracerosum-strigosum-littorale
Complex
Authors:
Jun Abe
,
Naoko Hirano
,
Ayumi Komiya
,
Naho Kanda
,
Anri Fujiwara
,
Sachie Hori
,
Yuki Tsuchikane
and
Hiroyuki Sekimoto
,
date:
05/20/2016,
view:
8340,
Q&A:
0
[Abstract] To prepare the knockdown transformants of the
Closterium peracerosum-strigosum-littorale
(
C. psl.
) complex, particle bombardment was applied with a newly constructed vector (pSA0104) with an endogenous constitutive promoter fused to a DNA fragment corresponding to an antisense strand of a target gene. Using a hygromycin ...
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