Mammalia

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    Protocols in Current Issue
    Delivery of AAV for Expression of Fluorescent Biosensors in Juvenile Mouse Hippocampus
    [Abstract]

    Genetically encoded fluorescent biosensors are versatile tools for studying brain metabolism and function in live tissue. The genetic information for these biosensors can be delivered into the brain by stereotaxic injection of engineered adeno-associated viruses (AAVs), which can selectively target different cell types depending on the capsid

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    In vivo Fluorescence Imaging of Extracellular ATP in the Mouse Cerebral Cortex with a Hybrid-type Optical Sensor
    [Abstract]

    Adenosine 5’-triphosphate (ATP) works as an extracellular signaling molecule for cells in the brain, such as neurons and glia. Cellular communication via release of ATP is involved in a range of processes required for normal brain functions, and aberrant communication is associated with brain disorders. To investigate the mechanisms

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    Development of a Chemical Reproductive Aging Model in Female Rats
    [Abstract]

    Women are born with an abundant but finite pool of ovarian follicles, which naturally and progressively decreased during their reproductive years until menstrual periods stop permanently (menopause). Perimenopause represents the transition from reproductive to non-reproductive life. It is usually characterized by neuroendocrine, metabolic and

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    Measuring Breathing Patterns in Mice Using Whole-body Plethysmography
    [Abstract] Respiratory dysfunction is among the main cause of severe and fatal pathologies worldwide. The use of effective experimental models and methodologies for the study of the pulmonary pathophysiology is necessary to prevent, control and cure these diseases. Plethysmography, a technique for the assessment of lung function, has been widely applied in ...
    Acute Cerebellar Slice Preparation Using a Tissue Chopper
    Authors:  Yubin Wang and Michel Baudry, date: 03/05/2019, view: 4335, Q&A: 0
    [Abstract] Acute cerebellar slices are widely used among neuroscientists to study the properties of excitatory and inhibitory synaptic transmission as well as intracellular signaling pathways involved in their regulation in cerebellum. The cerebellar cortex presents a well-organized circuitry, and several neuronal pathways can be stimulated and recorded ...
    Eye Drops for Delivery of Bioactive Compounds and BrdU to Stimulate Proliferation and Label Mitotically Active Cells in the Adult Rodent Retina
    Authors:  Cindy L. Linn, Sarah E. Webster and Mark K. Webster, date: 11/05/2018, view: 4122, Q&A: 0
    [Abstract] Eye drop treatments are typically used to apply drugs to the anterior structures of the eye. Recently, however, studies have demonstrated that eye drops can reach the retina in the back of the eye if pharmacological agents are carried in appropriate vehicles. Here, we introduce an eye drop procedure to deliver a drug (PNU-282987), in combination ...
    Testing Effects of Chronic Chemogenetic Neuronal Stimulation on Energy Balance by Indirect Calorimetry
    Authors:  Sangho Yu and Heike Münzberg, date: 04/20/2018, view: 4589, Q&A: 0
    [Abstract] The fundamental of neuroscience is to connect the firing of neurons to physiological and behavioral outcomes. Chemogenetics enables researchers to control the activity of a genetically defined population of neurons in vivo through the expression of designer receptor exclusively activated by designer drug (DREADD) in specific neurons and ...
    Spared Nerve Injury Model of Neuropathic Pain in Mice
    Authors:  Joseph Cichon, Linlin Sun and Guang Yang, date: 03/20/2018, view: 13565, Q&A: 1
    [Abstract] Experimental models of peripheral nerve injury have been developed to study mechanisms of neuropathic pain in living animals. The spared nerve injury (SNI) model in rodents is a partial denervation model, in which the common peroneal and tibial nerves are injured, producing consistent and reproducible tactile hypersensitivity in the skin territory ...
    Obtaining Acute Brain Slices
    Authors:  Thomas Papouin and Philip G. Haydon, date: 01/20/2018, view: 18565, Q&A: 1
    [Abstract] Obtaining acute brain slices for electrophysiology or amperometric recordings has become a routine procedure in most labs in the field of neuroscience. Yet, protocols describing the step by step process are scarce, in particular for routine acute preparations such as from the mouse hippocampus. Here we provide a detailed protocol for the ...
    Optogenetic Mapping of Synaptic Connections in Mouse Brain Slices to Define the Functional Connectome of Identified Neuronal Populations
    Authors:  Susana Mingote, Nao Chuhma and Stephen Rayport, date: 01/05/2017, view: 8943, Q&A: 0
    [Abstract] Functional connectivity in a neural circuit is determined by the strength, incidence, and neurotransmitter nature of its connections (Chuhma, 2015). Using optogenetics the functional synaptic connections between an identified population of neurons and defined postsynaptic target neurons may be measured systematically in order to determine the ...



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