Fungi

Categories

    Protocols in Current Issue
    Non-invasive Quantification of Cell Wall Porosity by Fluorescence Quenching Microscopy
    Authors:  Xiaohui Liu, Thomas Günther Pomorski and Johannes Liesche, date: 08/20/2019, view: 4061, Q&A: 0
    [Abstract] All bacteria, fungi and plant cells are surrounded by a cell wall. This complex network of polysaccharides and glycoproteins provides mechanical support, defines cell shape, controls cell growth and influences the exchange of substances between the cell and its surroundings. Despite its name, the cell wall is a flexible, dynamic structure. ...
    Determination of Boron Content Using a Simple and Rapid Miniaturized Curcumin Assay
    Authors:  Thotegowdanapalya C. Mohan and Alexandra M. E. Jones, date: 01/20/2018, view: 6672, Q&A: 0
    [Abstract] To determine boron quantity in soil, water and biological samples, several protocols are available. Colorimetric assays are the simplest and cheapest methods which can be used to determine boron concentration. However, published protocols do not give straightforward guidance for beginners to adopt these protocols for routine use in the laboratory. ...
    Freeze-fracture-etching Electron Microscopy for Facile Analysis of Yeast Ultrastructure
    Authors:  Takuma Tsuji and Toyoshi Fujimoto, date: 09/20/2017, view: 11574, Q&A: 0
    [Abstract] We describe a streamlined method that enables the quick observation of yeast ultrastructure by electron microscopy (EM). Yeast cells are high-pressure frozen, freeze-fractured to cut across the cytoplasm, and freeze-etched to sublimate ice in the cytosol and the organelle lumen. The cellular structures delineated by these procedures are coated by ...
    Measuring UV-induced Mutagenesis at the CAN1 Locus in Saccharomyces cerevisiae
    Authors:  Ildiko Unk and Andreea Daraba, date: 10/20/2014, view: 11060, Q&A: 1
    [Abstract] There are several methods to measure the capacity of yeast cell to respond to environmental impacts on their genome by mutating it. One frequently used method involves the detection of forward mutations in the CAN1 gene. The CAN1 gene encodes for an arginine permease that is responsible for the uptake of arginine and it can also ...
    Subcellular Fractionation Using Accudenz Gradient to Separate ER/Golgi in Yeast
    Author:  Zongtian Tong, date: 01/20/2012, view: 15138, Q&A: 0
    [Abstract] This protocol describes how to separate the endoplasmic reticulum (ER) and Golgi apparatus in yeast cells using a subcellular fractionation approach with an Accudenz gradient.



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.