Prokaryotes

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    Protocols in Current Issue
    Preparation of Pneumococcal Proteins for Western Blot Analysis
    Authors:  Maria João Frias, José Melo-Cristino and Mário Ramirez, date: 07/05/2013, view: 8354, Q&A: 0
    [Abstract] This protocol was developed in a study aimed to determine the cellular localization of the lysin of pneumococcal phage SV1 (Frias et al., 2013). We obtained proteins from the surface of Streptococcus pneumoniae by elution with choline or those secreted to the medium. The analysis by Western blot of these fractions allowed us to ...



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