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Preparation of Pneumococcal Proteins for Western Blot Analysis
Authors:
Maria João Frias
,
José Melo-Cristino
and
Mário Ramirez
,
date:
07/05/2013,
view:
8354,
Q&A:
0
[Abstract] This protocol was developed in a study aimed to determine the cellular localization of the lysin of pneumococcal phage SV1 (Frias
et al
., 2013). We obtained proteins from the surface of
Streptococcus pneumoniae
by elution with choline or those secreted to the medium. The analysis by Western blot of these fractions allowed us to ...
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