Generation of Tumour-stroma Minispheroids for Drug Efficacy Testing
Authors: Mark Watters and
Eva Szegezdi,
date: 01/05/2017,
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[Abstract] The three-dimensional organisation of cells in a tissue and their interaction with adjacent cells and extracellular matrix is a key determinant of cellular responses, including how tumour cells respond to stress conditions or therapeutic drugs (Elliott and Yuan, 2011). In vivo, tumour cells are embedded in a stroma formed primarily by ...
Three Dimensional Spheroid Co-culture Invasion Assay
Authors: Eldo T Verghese and
Thomas A Hughes,
date: 01/05/2015,
view: 11977,
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[Abstract] The assay was developed to investigate the impact of stromal cells of different types (in our case breast cancer associated fibroblasts stably manipulated to modify expression of genes of interest) on the invasive capacity of epithelial cancer cells (in our case breast cancer cell lines) (Verghese et al., 2013). Typical two dimensional ...