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Determination of Polyhydroxybutyrate (PHB) Content in
Ralstonia eutropha
Using Gas Chromatography and Nile Red Staining
Authors:
Janina R. Juengert
,
Stephanie Bresan
and
Dieter Jendrossek
,
date:
03/05/2018,
view:
13515,
Q&A:
0
[Abstract]
Ralstonia eutropha
H16 produces and mobilizes (re-utilizes) intracellular polyhydroxybutyrate (PHB) granules during growth. This protocol describes the visualization of intracellular Nile red stained PHB granules and the quantification of PHB by gas chromatography. Our first method describes how to analyze PHB granules by fluorescence ...
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