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Isolation of Latex Bead Phagosomes from
Dictyostelium
for
in vitro
Functional Assays
Authors:
Ashwin D’Souza
,
Paulomi Sanghavi
,
Ashim Rai
,
Divya Pathak
and
Roop Mallik
,
date:
12/05/2016,
view:
8564,
Q&A:
0
[Abstract] We describe a protocol to purify latex bead phagosomes (LBPs) from
Dictyostelium
cells. These can be later used for various
in vitro
functional assays. For instance, we use these LBPs to understand the microtubule motor-driven transport on
in vitro
polymerized microtubules. Phagosomes are allowed to mature for defined ...
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